View source for 5ajt


==Crystal structure of ligand-free phosphoribohydrolase lonely guy from Claviceps purpurea==
<StructureSection load='5ajt' size='340' side='right'caption='[[5ajt]], [[Resolution|resolution]] 2.43&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5ajt]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Claviceps_purpurea Claviceps purpurea]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5AJT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5AJT FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.43&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=TAR:D(-)-TARTARIC+ACID'>TAR</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5ajt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ajt OCA], [https://pdbe.org/5ajt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5ajt RCSB], [https://www.ebi.ac.uk/pdbsum/5ajt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5ajt ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/M1VUY5_CLAP2 M1VUY5_CLAP2] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The recently discovered cytokinin-specific phosphoribohydrolase 'Lonely Guy' (LOG) is a key enzyme of cytokinin biosynthesis, converting inactive cytokinin nucleotides into biologically active free-bases. We have determined the crystal structures of LOG from C. purpurea (cpLOG) and its complex with the enzymatic product phosphoribose. The structures reveal a dimeric arrangement of Rossmann folds, with the ligands bound to large pockets at the interface between cpLOG monomers. Structural comparisons highlight the homology of cpLOG to putative lysine decarboxylases. Extended sequence analysis enabled identification of a distinguishing LOG sequence signature. Taken together, our data suggest phosphoribohydrolase activity for several proteins of unknown function. This article is protected by copyright. All rights reserved.

The three-dimensional Structure of "Lonely Guy" from Claviceps purpurea provides insights into the phosphoribohydrolase function of Rossmann fold-containing lysine decarboxylase-like proteins.,Dzurova L, Forneris F, Savino S, Galuszka P, Vrabka J, Frebort I Proteins. 2015 May 25. doi: 10.1002/prot.24835. PMID:26010010<ref>PMID:26010010</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5ajt" style="background-color:#fffaf0;"></div>
== References ==
<references/>
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</StructureSection>
[[Category: Claviceps purpurea]]
[[Category: Large Structures]]
[[Category: Dzurova L]]
[[Category: Forneris F]]
[[Category: Savino S]]