View source for 5avm


==Crystal structures of 5-aminoimidazole ribonucleotide (AIR) synthetase, PurM, from Thermus thermophilus==
<StructureSection load='5avm' size='340' side='right'caption='[[5avm]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5avm]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus_HB8 Thermus thermophilus HB8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5AVM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5AVM FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5avm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5avm OCA], [https://pdbe.org/5avm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5avm RCSB], [https://www.ebi.ac.uk/pdbsum/5avm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5avm ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q5SLC6_THET8 Q5SLC6_THET8] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Crystal structures of 5-aminoimidazole ribonucleotide (AIR) synthetase, also known as PurM, from Thermus thermophilus (Tt) and Geobacillus kaustophilus (Gk) were determined. For TtPurM, the maximum resolution was 2.2 A and the space group was P21212 with four dimers in an asymmetric unit. For GkPurM, the maximum resolution was 2.2 A and the space group was P21212 with one monomer in asymmetric unit. The biological unit is dimer for both TtPurM and GkPurM and the dimer structures were similar to previously determined structures of PurM in general. For TtPurM, approximately 50 residues at the amino terminal were disordered in the crystal structure whereas, for GkPurM, the corresponding region covered the ATP-binding site forming an alpha helix in part, suggesting that the N-terminal region of PurM changes its conformation upon binding of ligands. FGAM binding site was predicted by the docking simulation followed by the MD simulation based on the SO4 2- binding site found in the crystal structure of TtPurM.

Crystal structures and ligand binding of PurM proteins from Thermus thermophilus and Geobacillus kaustophilus.,Kanagawa M, Baba S, Watanabe Y, Nakagawa N, Ebihara A, Kuramitsu S, Yokoyama S, Sampei GI, Kawai G J Biochem. 2015 Oct 29. pii: mvv107. PMID:26515187<ref>PMID:26515187</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5avm" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Thermus thermophilus HB8]]
[[Category: Baba S]]
[[Category: Ebihara A]]
[[Category: Kanagawa M]]
[[Category: Kawai G]]
[[Category: Nakagawa N]]
[[Category: Sampei G]]
[[Category: Watanabe Y]]