View source for 5azi


==Crystal structure of glycerol kinase from Trypanosoma brucei gambiense complexed with 4NP==
<StructureSection load='5azi' size='340' side='right'caption='[[5azi]], [[Resolution|resolution]] 2.45&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5azi]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Trypanosoma_brucei_gambiense Trypanosoma brucei gambiense]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5AZI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5AZI FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.45&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=4NP:4-NITROPHENYL+PHOSPHATE'>4NP</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=TPO:PHOSPHOTHREONINE'>TPO</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5azi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5azi OCA], [https://pdbe.org/5azi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5azi RCSB], [https://www.ebi.ac.uk/pdbsum/5azi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5azi ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/D3KVM3_TRYBG D3KVM3_TRYBG] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
BACKGROUND: In general, glycerol kinases (GKs) are transferases that catalyze phospho group transfer from ATP to glycerol, and the mechanism was suggested to be random bi-bi. The reverse reaction i.e. phospho transfer from glycerol 3-phosphate (G3P) to ADP is only physiologically feasible by the African trypanosome GK. In contrast to other GKs the mechanism of Trypanosoma brucei gambiense glycerol kinase (TbgGK) was shown to be in an ordered fashion, and proceeding via autophosphorylation. From the unique reaction mechanism of TbgGK, we envisaged its potential to possess phosphatase activity in addition to being a kinase. METHODS: Our hypothesis was tested by spectrophotometric and LC-MS/MS analyses using paranitrophenyl phosphate (pNPP) and TbgGK's natural substrate, G3P respectively. Furthermore, protein X-ray crystallography and site-directed mutagenesis were performed to examine pNPP binding, catalytic residues, and the possible reaction mechanism. RESULTS: In addition to its widely known and expected phosphotransferase (class II) activity, TbgGK can efficiently facilitate the hydrolytic cleavage of phosphoric anhydride bonds (a class III property). This phosphatase activity followed the classical Michaelis-Menten pattern and was competitively inhibited by ADP and G3P, suggesting a common catalytic site for both activities (phosphatase and kinase). The structure of the TGK-pNPP complex, and structure-guided mutagenesis implicated T276 to be important for the catalysis. Remarkably, we captured a crystallographic molecular snapshot of the phosphorylated T276 reaction intermediate. CONCLUSION: We conclude that TbgGK has both kinase and phosphatase activities. GENERAL SIGNIFICANCE: This is the first report on a bifunctional kinase/phosphatase enzyme among members of the sugar kinase family.

Glycerol kinase of African trypanosomes possesses an intrinsic phosphatase activity.,Balogun EO, Inaoka DK, Shiba T, Tokuoka SM, Tokumasu F, Sakamoto K, Kido Y, Michels PAM, Watanabe YI, Harada S, Kita K Biochim Biophys Acta Gen Subj. 2017 Nov;1861(11 Pt A):2830-2842. doi:, 10.1016/j.bbagen.2017.07.028. Epub 2017 Aug 2. PMID:28778484<ref>PMID:28778484</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5azi" style="background-color:#fffaf0;"></div>

==See Also==
*[[Glycerol kinase|Glycerol kinase]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Trypanosoma brucei gambiense]]
[[Category: Balogun EO]]
[[Category: Harada S]]
[[Category: Inaoka DK]]
[[Category: Kita K]]
[[Category: Michels PAM]]
[[Category: Sakamoto K]]
[[Category: Shiba T]]
[[Category: Tokumasu F]]
[[Category: Tokuoka SM]]