View source for 5b0b


==Polyketide cyclase OAC from Cannabis sativa, I7F mutant==
<StructureSection load='5b0b' size='340' side='right'caption='[[5b0b]], [[Resolution|resolution]] 2.19&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5b0b]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Cannabis_sativa Cannabis sativa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5B0B OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5B0B FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.187&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5b0b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5b0b OCA], [https://pdbe.org/5b0b PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5b0b RCSB], [https://www.ebi.ac.uk/pdbsum/5b0b PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5b0b ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/OLIAC_CANSA OLIAC_CANSA] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
In polyketide biosynthesis, ring formation is one of the key diversification steps. Olivetolic acid cyclase (OAC) from Cannabis sativa, involved in cannabinoid biosynthesis, is the only known plant polyketide cyclase. In addition, it is the only functionally characterized plant alpha+beta barrel (DABB) protein that catalyzes the C2-C7 aldol cyclization of the linear pentyl tetra-beta-ketide CoA as the substrate, to generate olivetolic acid (OA). Herein, we solved the OAC apo and OAC-OA complex binary crystal structures at 1.32 and 1.70 A resolutions, respectively. The crystal structures revealed that the enzyme indeed belongs to the DABB superfamily, as previously proposed, and possesses a unique active-site cavity containing the pentyl-binding hydrophobic pocket and the polyketide binding site, which have never been observed among the functionally and structurally characterized bacterial polyketide cyclases. Furthermore, site-directed mutagenesis studies indicated that Tyr72 and His78 function as acid/base catalysts at the catalytic center. Structural and/or functional studies of OAC suggested that the enzyme lacks thioesterase and aromatase activities. These observations demonstrated that OAC employs unique catalytic machinery utilizing acid/base catalytic chemistry for the formation of the precursor of OA. The structural and functional insights obtained in this work thus provide the foundation for analyses of the plant polyketide cyclases that will be discovered in the future. DATA DEPOSITION: Structural data reported in this paper are available in the Protein Data Bank under the accession numbers 5B08 for the OAC apo, 5B09 for the OAC-OA binary complex and 5B0A, 5B0B, 5B0C, 5B0D, 5B0E, 5B0F and 5B0G for the OAC His5Q, Ile7F, Tyr27F, Tyr27W, Val59M, Tyr72F and His78S mutant enzymes, respectively.

Structural basis for olivetolic acid formation by a polyketide cyclase from Cannabis sativa.,Yang X, Matsui T, Kodama T, Mori T, Zhou X, Taura F, Noguchi H, Abe I, Morita H FEBS J. 2016 Mar;283(6):1088-106. doi: 10.1111/febs.13654. Epub 2016 Feb 2. PMID:26783002<ref>PMID:26783002</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5b0b" style="background-color:#fffaf0;"></div>
== References ==
<references/>
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</StructureSection>
[[Category: Cannabis sativa]]
[[Category: Large Structures]]
[[Category: Abe I]]
[[Category: Matsui T]]
[[Category: Mori T]]
[[Category: Morita H]]
[[Category: Yang X]]