View source for 5b3i


==Homo-dimeric structure of cytochrome c' from Thermophilic Hydrogenophilus thermoluteolus==
<StructureSection load='5b3i' size='340' side='right'caption='[[5b3i]], [[Resolution|resolution]] 1.89&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5b3i]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Hydrogenophilus_thermoluteolus Hydrogenophilus thermoluteolus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5B3I OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5B3I FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.89&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEC:HEME+C'>HEC</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5b3i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5b3i OCA], [https://pdbe.org/5b3i PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5b3i RCSB], [https://www.ebi.ac.uk/pdbsum/5b3i PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5b3i ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/F7J213_HYDTE F7J213_HYDTE] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Thermophilic Hydrogenophilus thermoluteolus cytochrome c' (PHCP) exhibits higher thermal stability than a mesophilic counterpart, Allochromatium vinosum cytochrome c' (AVCP), which has a homo-dimeric structure and ligand-binding ability. To understand the thermal stability mechanism and ligand-binding ability of the thermally stable PHCP protein, the crystal structure of PHCP was first determined. It formed a homo-dimeric structure, the main chain root mean square deviation (rmsd) value between PHCP and AVCP being 0.65 A. In the PHCP structure, six specific residues appeared to strengthen the heme-related and subunit-subunit interactions, which were not conserved in the AVCP structure. PHCP variants having altered subunit-subunit interactions were more severely destabilized than ones having altered heme-related interactions. The PHCP structure further revealed a ligand-binding channel and a penta-coordinated heme, as observed in the AVCP protein. A spectroscopic study clearly showed that some ligands were bound to the PHCP protein. It is concluded that the dimeric PHCP from the thermophile is effectively stabilized through heme-related and subunit-subunit interactions with conservation of the ligand-binding ability. BRIEF SUMMARY: We report the X-ray crystal structure of cytochrome c' (PHCP) from thermophilic Hydrogenophilus thermoluteolus. The high thermal stability of PHCP was attributed to heme-related and subunit-subunit interactions, which were confirmed by a mutagenesis study. The ligand-binding ability of PHCP was examined by spectrophotometry. PHCP acquired the thermal stability with conservation of the ligand-binding ability. This study furthers the understanding of the stability and function of cytochromes c.

Structural and functional insights into thermally stable cytochrome c' from a thermophile.,Fujii S, Oki H, Kawahara K, Yamane D, Yamanaka M, Maruno T, Kobayashi Y, Masanari M, Wakai S, Nishihara H, Ohkubo T, Sambongi Y Protein Sci. 2017 Jan 18. doi: 10.1002/pro.3120. PMID:28097774<ref>PMID:28097774</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5b3i" style="background-color:#fffaf0;"></div>

==See Also==
*[[Cytochrome C 3D structures|Cytochrome C 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Hydrogenophilus thermoluteolus]]
[[Category: Large Structures]]
[[Category: Fujii S]]
[[Category: Kawahara K]]
[[Category: Kobayashi Y]]
[[Category: Maruno T]]
[[Category: Masanari M]]
[[Category: Nishihara H]]
[[Category: Ohkubo T]]
[[Category: Oki H]]
[[Category: Sambongi Y]]
[[Category: Wakai S]]
[[Category: Yamanaka M]]
[[Category: Yamane D]]