View source for 5bob


==Crystal Structure of the Meningitis Pathogen Streptococcus suis adhesion Fhb==
<StructureSection load='5bob' size='340' side='right'caption='[[5bob]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5bob]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_suis_05ZYH33 Streptococcus suis 05ZYH33]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5BOB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5BOB FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5bob FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5bob OCA], [https://pdbe.org/5bob PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5bob RCSB], [https://www.ebi.ac.uk/pdbsum/5bob PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5bob ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A4VT01_STRSY A4VT01_STRSY] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Fhb is a surface virulence protein from Streptococcus suis, which could aid bacterial evasion of host innate immune defense by recruiting complement regulator factor H to inactivate C3b deposited on bacterial surface in blood. Here we successfully expressed and purified the N terminal domain of Fhb (N-Fhb) and obtained crystals of the N-Fhb by sitting-drop vapor diffusion method with a resolution of 1.50 A. The crystals belong to space group C2 with unit cell parameters a = 127.1 A, b = 77.3 A, c = 131.6 A, alpha = 90 degrees , beta = 115.9 degrees , gamma = 90 degrees . The structure of N-Fhb was determined by SAD method and the core structure of N-Fhb is a beta sandwich. We speculated that binding of Fhb to human factor H may be mainly mediated by surface amino acids with negative charges.

Expression, purification, crystallization and structure determination of the N terminal domain of Fhb, a factor H binding protein from Streptococcus suis.,Zhang C, Yu Y, Yang M, Jiang Y Biochem Biophys Res Commun. 2015 Oct 23;466(3):413-7. doi:, 10.1016/j.bbrc.2015.09.040. Epub 2015 Sep 10. PMID:26365348<ref>PMID:26365348</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5bob" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Streptococcus suis 05ZYH33]]
[[Category: Jiang Y]]
[[Category: Yu Y]]
[[Category: Zhang C]]