View source for 5bpj


==All Three Ca(2+)-binding Loops of Light-sensitive Ctenophore Photoprotein Berovin Bind Magnesium Ions: The Spatial Structure of Mg(2+)-loaded Apo-berovin==
<StructureSection load='5bpj' size='340' side='right'caption='[[5bpj]], [[Resolution|resolution]] 1.76&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5bpj]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Beroe_abyssicola Beroe abyssicola]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5BPJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5BPJ FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.756&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5bpj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5bpj OCA], [https://pdbe.org/5bpj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5bpj RCSB], [https://www.ebi.ac.uk/pdbsum/5bpj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5bpj ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/H8ZZK1_BERAB H8ZZK1_BERAB] 
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== Publication Abstract from PubMed ==
Light-sensitive photoprotein berovin accounts for a bright bioluminescence of ctenophore Beroe abyssicola. Berovin is functionally identical to the well-studied Ca(2+)-regulated photoproteins of jellyfish, however in contrast to those it is extremely sensitive to the visible light. Berovin contains three EF-hand Ca(2+)-binding sites and consequently belongs to a large family of the EF-hand Ca(2+)-binding proteins. Here we report the spatial structure of apo-berovin with bound Mg(2+) determined at 1.75A. The magnesium ion is found in each functional EF-hand loop of a photoprotein and coordinated by oxygen atoms donated by the side-chain groups of aspartate, carbonyl groups of the peptide backbone, or hydroxyl group of serine with characteristic oxygen-Mg(2+) distances. As oxygen supplied by the side-chain of the twelfth residue of all Ca(2+)-binding loops participates in the magnesium ion coordination, it was suggested that Ca(2+)-binding loops of berovin belong to the mixed Ca(2+)/Mg(2+) rather than Ca(2+)-specific type. In addition, we report an effect of physiological concentration of Mg(2+) on bioluminescence of berovin (sensitivity to Ca(2+), rapid-mixed kinetics, light-sensitivity, thermostability, and apo-berovin conversion into active protein). The different impact of physiological concentration of Mg(2+) on berovin bioluminescence as compared to hydromedusan photoproteins was attributed to different affinities of the Ca(2+)-binding sites of these photoproteins to Mg(2+).

All Ca(2+)-binding loops of light-sensitive ctenophore photoprotein berovin bind magnesium ions: The spatial structure of Mg(2+)-loaded apo-berovin.,Burakova LP, Natashin PV, Malikova NP, Niu F, Pu M, Vysotski ES, Liu ZJ J Photochem Photobiol B. 2016 Jan;154:57-66. doi:, 10.1016/j.jphotobiol.2015.11.012. Epub 2015 Dec 2. PMID:26690016<ref>PMID:26690016</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5bpj" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Beroe abyssicola]]
[[Category: Large Structures]]
[[Category: Burakova LP]]
[[Category: Liu Z-J]]
[[Category: Malikova NP]]
[[Category: Natashin PV]]
[[Category: Niu F]]
[[Category: Vysotski ES]]