View source for 5cqg


==Structure of Tribolium telomerase in complex with the highly specific inhibitor BIBR1532==
<StructureSection load='5cqg' size='340' side='right'caption='[[5cqg]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5cqg]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Tribolium_castaneum Tribolium castaneum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5CQG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5CQG FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=55C:2-{[(2E)-3-(NAPHTHALEN-2-YL)BUT-2-ENOYL]AMINO}BENZOIC+ACID'>55C</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5cqg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5cqg OCA], [https://pdbe.org/5cqg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5cqg RCSB], [https://www.ebi.ac.uk/pdbsum/5cqg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5cqg ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q0QHL8_TRICA Q0QHL8_TRICA] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
BIBR1532 is a highly specific telomerase inhibitor, although the molecular basis for inhibition is unknown. Here we present the crystal structure of BIBR1532 bound to Tribolium castaneum catalytic subunit of telomerase (tcTERT). BIBR1532 binds to a conserved hydrophobic pocket (FVYL motif) on the outer surface of the thumb domain. The FVYL motif is near TRBD residues that bind the activation domain (CR4/5) of hTER. RNA binding assays show that the human TERT (hTERT) thumb domain binds the P6.1 stem loop of CR4/5 in vitro. hTERT mutations of the FVYL pocket alter wild-type CR4/5 binding and cause telomere attrition in cells. Furthermore, the hTERT FVYL mutations V1025F, N1028H, and V1090M are implicated in dyskeratosis congenita and aplastic anemia, further supporting the biological and clinical relevance of this novel motif. We propose that CR4/5 contacts with the telomerase thumb domain contribute to telomerase ribonucleoprotein assembly and promote enzymatic activity.

Structural Basis of Telomerase Inhibition by the Highly Specific BIBR1532.,Bryan C, Rice C, Hoffman H, Harkisheimer M, Sweeney M, Skordalakes E Structure. 2015 Oct 6;23(10):1934-1942. doi: 10.1016/j.str.2015.08.006. Epub 2015, Sep 10. PMID:26365799<ref>PMID:26365799</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5cqg" style="background-color:#fffaf0;"></div>

==See Also==
*[[Telomerase 3D structures|Telomerase 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Tribolium castaneum]]
[[Category: Bryan C]]
[[Category: Harkisheimer M]]
[[Category: Hoffman H]]
[[Category: Rice C]]
[[Category: Skordalakes E]]
[[Category: Sweeney M]]