View source for 5ctu


==Crystal structure of the ATP binding domain of S. aureus GyrB complexed with a fragment==
<StructureSection load='5ctu' size='340' side='right'caption='[[5ctu]], [[Resolution|resolution]] 1.45&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5ctu]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5CTU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5CTU FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.45&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=54X:5-(THIOPHEN-2-YL)THIENO[2,3-D]PYRIMIDIN-4(1H)-ONE'>54X</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5ctu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ctu OCA], [https://pdbe.org/5ctu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5ctu RCSB], [https://www.ebi.ac.uk/pdbsum/5ctu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5ctu ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/GYRB_STAAU GYRB_STAAU] DNA gyrase negatively supercoils closed circular double-stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings.[HAMAP-Rule:MF_01898]
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Inhibitors of the ATPase function of bacterial DNA gyrase, located in the GyrB subunit and its related ParE subunit in topoisomerase IV, have demonstrated antibacterial activity. In this study we describe an NMR fragment-based screening effort targeting Staphylococcus aureus GyrB that identified several attractive and novel starting points with good ligand efficiency. Fragment hits were further characterized using NMR binding studies against full-length S. aureus GyrB and Escherichia coli ParE. X-ray co-crystal structures of select fragment hits confirmed binding and suggested a path for medicinal chemistry optimization. The identification, characterization, and elaboration of one of these fragment series to a 0.265muM inhibitor is described herein.

Fragment-based discovery of DNA gyrase inhibitors targeting the ATPase subunit of GyrB.,Mesleh MF, Cross JB, Zhang J, Kahmann J, Andersen OA, Barker J, Cheng RK, Felicetti B, Wood M, Hadfield AT, Scheich C, Moy TI, Yang Q, Shotwell J, Nguyen K, Lippa B, Dolle R, Ryan MD Bioorg Med Chem Lett. 2016 Jan 6. pii: S0960-894X(16)30009-9. doi:, 10.1016/j.bmcl.2016.01.009. PMID:26786695<ref>PMID:26786695</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5ctu" style="background-color:#fffaf0;"></div>

==See Also==
*[[Gyrase 3D Structures|Gyrase 3D Structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Staphylococcus aureus]]
[[Category: Andersen OA]]
[[Category: Barker J]]
[[Category: Cheng RK]]
[[Category: Cross JB]]
[[Category: Felicetti B]]
[[Category: Kahmann J]]
[[Category: Lippa B]]
[[Category: Mesleh M]]
[[Category: Ryan MD]]
[[Category: Scheich C]]
[[Category: Wood M]]
[[Category: Yang Q]]
[[Category: Zhang J]]