View source for 5cuw


==Crystal structure of Sortase E1 from Streptomyces coelicolor with tripeptide in the active site==
<StructureSection load='5cuw' size='340' side='right'caption='[[5cuw]], [[Resolution|resolution]] 1.89&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5cuw]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_coelicolor_A3(2) Streptomyces coelicolor A3(2)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5CUW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5CUW FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.887&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5cuw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5cuw OCA], [https://pdbe.org/5cuw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5cuw RCSB], [https://www.ebi.ac.uk/pdbsum/5cuw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5cuw ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/SRTE1_STRCO SRTE1_STRCO] Transpeptidase that anchors surface proteins to the cell wall. Recognizes both Leu-Ala-x-Thr-Gly and Leu-Pro-x-Thr-Gly, with a preference for the former. Unlike the S.aureus sortase it cleaves not only the Thr-Gly motif but also the Ala-X bond; Ala-Glu and Ala-His bonds are better substrates than the Thr-Gly motif in vitro (PubMed:22296345, PubMed:27936128). Among its possible substrates are the chaplins ChpA, ChpB and ChpC; this enzyme is less important for ChpC attachment than is SrtE2. A double knockout mutant of srtE1 and srtE2 shows a developmental defect in aerial hyphae formation more dramatic than that due to chaplin deletion (PubMed:22296345).<ref>PMID:22296345</ref> <ref>PMID:27936128</ref> 
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Cascio D]]
[[Category: Clubb RT]]
[[Category: Kattke MD]]
[[Category: Sawaya MR]]