View source for 5cxc


==Structure of Ytm1 bound to the C-terminal domain of Erb1 in P 65 2 2 space group==
<StructureSection load='5cxc' size='340' side='right'caption='[[5cxc]], [[Resolution|resolution]] 3.10&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5cxc]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Chaetomium_thermophilum Chaetomium thermophilum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5CXC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5CXC FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.1&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5cxc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5cxc OCA], [https://pdbe.org/5cxc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5cxc RCSB], [https://www.ebi.ac.uk/pdbsum/5cxc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5cxc ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/YTM1_CHATD YTM1_CHATD] Component of the NOP7 complex, which is required for maturation of the 25S and 5.8S ribosomal RNAs and formation of the 60S ribosome.[HAMAP-Rule:MF_03029]
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== Publication Abstract from PubMed ==
Ribosome biogenesis is one of the most essential pathways in eukaryotes although it is still not fully characterized. Given the importance of this process in proliferating cells, it is obvious that understanding the macromolecular details of the interactions that take place between the assembly factors, ribosomal proteins and nascent pre-rRNAs is essentially required for the development of new non-genotoxic treatments for cancer. Herein, we have studied the association between the WD40-repeat domains of Erb1 and Ytm1 proteins. These are essential factors for the biogenesis of 60S ribosomal subunits in eukaryotes that form a heterotrimeric complex together with the also essential Nop7 protein. We provide the crystal structure of a dimer formed by the C-terminal part of Erb1 and Ytm1 from Chaetomium thermophilum at 2.1 A resolution. Using a multidisciplinary approach we show that the beta-propeller domains of these proteins interact in a novel manner that leads to a high-affinity binding. We prove that a point mutation within the interface of the complex impairs the interaction between the two proteins and negatively affects growth and ribosome production in yeast. Our study suggests insights into the association of the Erb1-Ytm1 dimer with pre-ribosomal particles.

The structure of Erb1-Ytm1 complex reveals the functional importance of a high-affinity binding between two beta-propellers during the assembly of large ribosomal subunits in eukaryotes.,Wegrecki M, Rodriguez-Galan O, de la Cruz J, Bravo J Nucleic Acids Res. 2015 Dec 15;43(22):11017-30. doi: 10.1093/nar/gkv1043. Epub, 2015 Oct 17. PMID:26476442<ref>PMID:26476442</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 5cxc" style="background-color:#fffaf0;"></div>

==See Also==
*[[Ribosome biogenesis protein 3D structures|Ribosome biogenesis protein 3D structures]]
== References ==
<references/>
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</StructureSection>
[[Category: Chaetomium thermophilum]]
[[Category: Large Structures]]
[[Category: Bravo J]]
[[Category: Wegrecki M]]