View source for 5cy0


==Total Chemical Synthesis, Covalent Structure Verification, and X-ray Structure of Bioactive Ts3 Toxin by Racemic Protein Crystallography==
<StructureSection load='5cy0' size='340' side='right'caption='[[5cy0]], [[Resolution|resolution]] 1.93&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5cy0]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Tityus_serrulatus Tityus serrulatus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5CY0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5CY0 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.93&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5cy0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5cy0 OCA], [https://pdbe.org/5cy0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5cy0 RCSB], [https://www.ebi.ac.uk/pdbsum/5cy0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5cy0 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/SCX3_TITSE SCX3_TITSE] Binds voltage-dependently at site-3 of sodium channels (Nav) and inhibits the inactivation of the activated channels, thereby blocking neuronal transmission. This toxin is active against mammals (mice).<ref>PMID:15051403</ref> <ref>PMID:15249424</ref> <ref>PMID:2536799</ref> 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Ts3 is an alpha scorpion toxin from the venom of the Brazilian scorpion Tityus serrulatus. Ts3 binds to the domain IV voltage sensor of voltage-gated sodium channels (Nav ) and slows down their fast inactivation. The covalent structure of the Ts3 toxin is uncertain, and the structure of the folded protein molecule is unknown. Herein, we report the total chemical synthesis of four candidate Ts3 toxin protein molecules and the results of structure-activity studies that enabled us to establish the covalent structure of biologically active Ts3 toxin. We also report the synthesis of the mirror image form of the Ts3 protein molecule, and the use of racemic protein crystallography to determine the folded (tertiary) structure of biologically active Ts3 toxin by X-ray diffraction.

Elucidation of the Covalent and Tertiary Structures of Biologically Active Ts3 Toxin.,Dang B, Kubota T, Mandal K, Correa AM, Bezanilla F, Kent SB Angew Chem Int Ed Engl. 2016 May 31. doi: 10.1002/anie.201603420. PMID:27244051<ref>PMID:27244051</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5cy0" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Tityus serrulatus]]
[[Category: Bezanilla F]]
[[Category: Correa AM]]
[[Category: Dang B]]
[[Category: Kent SB]]
[[Category: Kubota T]]
[[Category: Mandal K]]