View source for 5ddk


==Succinyl-CoA:acetate CoA-transferase (AarCH6-N347A) in complex with CoA==
<StructureSection load='5ddk' size='340' side='right'caption='[[5ddk]], [[Resolution|resolution]] 2.13&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5ddk]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Acetobacter_aceti_1023 Acetobacter aceti 1023]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5DDK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5DDK FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.127&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=COA:COENZYME+A'>COA</scene>, <scene name='pdbligand=IMD:IMIDAZOLE'>IMD</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5ddk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ddk OCA], [https://pdbe.org/5ddk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5ddk RCSB], [https://www.ebi.ac.uk/pdbsum/5ddk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5ddk ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/SCACT_ACEAC SCACT_ACEAC] Utilizes succinyl-CoA to convert toxic acetate to acetyl-CoA and succinate. Required for growth on acetic acid and for resistance to high levels of acetic acid. Has also low activity with acetoacetate as substrate.<ref>PMID:18502856</ref> <ref>PMID:23030530</ref> <ref>PMID:18502856</ref> [REFERENCE:3]
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Coenzyme A (CoA)-transferases catalyze the reversible transfer of CoA from acyl-CoA thioesters to free carboxylates. Class I CoA-transferases produce acylglutamyl anhydride intermediates that undergo attack by CoA thiolate on either the internal or external carbonyl carbon atoms, forming distinct tetrahedral intermediates &lt;3 A apart. In this study, crystal structures of succinyl-CoA:acetate CoA-transferase (AarC) from Acetobacter aceti are used to examine how the Asn347 carboxamide stabilizes the internal oxyanion intermediate. A structure of the active mutant AarC-N347A bound to CoA revealed both solvent replacement of the missing contact and displacement of the adjacent Glu294, indicating that Asn347 both polarizes and orients the essential glutamate. AarC was crystallized with the nonhydrolyzable acetyl-CoA (AcCoA) analog dethiaacetyl-CoA (1a) in an attempt to trap a closed enzyme complex containing a stable analog of the external oxyanion intermediate. One active site contained an acetylglutamyl anhydride adduct and truncated 1a, an unexpected result hinting at an unprecedented cleavage of the ketone moiety in 1a. Solution studies confirmed that 1a decomposition is accompanied by production of near-stoichiometric acetate, in a process that seems to depend on microbial contamination but not AarC. A crystal structure of AarC bound to the postulated 1a truncation product (2a) showed complete closure of one active site per dimer but no acetylglutamyl anhydride, even with acetate added. These findings suggest that an activated acetyl donor forms during 1a decomposition; a working hypothesis involving ketone oxidation is offered. The ability of 2a to induce full active site closure furthermore suggests that it subverts a system used to impede inappropriate active site closure on unacylated CoA.

Functional Dissection of the Bipartite Active Site of the Class I Coenzyme A (CoA)-Transferase Succinyl-CoA:Acetate CoA-Transferase.,Murphy JR, Mullins EA, Kappock TJ Front Chem. 2016 May 23;4:23. doi: 10.3389/fchem.2016.00023. eCollection 2016. PMID:27242998<ref>PMID:27242998</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5ddk" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Acetobacter aceti 1023]]
[[Category: Large Structures]]
[[Category: Kappock TJ]]
[[Category: Mullins EA]]
[[Category: Murphy JR]]