View source for 5dol


==Crystal structure of YabA amino-terminal domain from Bacillus subtilis==
<StructureSection load='5dol' size='340' side='right'caption='[[5dol]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5dol]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis_subsp._subtilis_str._168 Bacillus subtilis subsp. subtilis str. 168]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5DOL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5DOL FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5dol FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5dol OCA], [https://pdbe.org/5dol PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5dol RCSB], [https://www.ebi.ac.uk/pdbsum/5dol PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5dol ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/YABA_BACSU YABA_BACSU] Involved in initiation control of chromosome replication.<ref>PMID:12060778</ref> 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
YabA negatively regulates initiation of DNA replication in low-GC Gram-positive bacteria. The protein exerts its control through interactions with the initiator protein DnaA and the sliding clamp DnaN. Here, we combined X-ray crystallography, X-ray scattering (SAXS), modeling and biophysical approaches, with in vivo experimental data to gain insight into YabA function. The crystal structure of the N-terminal domain (NTD) of YabA solved at 2.7 A resolution reveals an extended alpha-helix that contributes to an intermolecular four-helix bundle. Homology modeling and biochemical analysis indicates that the C-terminal domain (CTD) of YabA is a small Zn-binding domain. Multi-angle light scattering and SAXS demonstrate that YabA is a tetramer in which the CTDs are independent and connected to the N-terminal four-helix bundle via flexible linkers. While YabA can simultaneously interact with both DnaA and DnaN, we found that an isolated CTD can bind to either DnaA or DnaN, individually. Site-directed mutagenesis and yeast-two hybrid assays identified DnaA and DnaN binding sites on the YabA CTD that partially overlap and point to a mutually exclusive mode of interaction. Our study defines YabA as a novel structural hub and explains how the protein tetramer uses independent CTDs to bind multiple partners to orchestrate replication initiation in the bacterial cell.

Tetramerization and interdomain flexibility of the replication initiation controller YabA enables simultaneous binding to multiple partners.,Felicori L, Jameson KH, Roblin P, Fogg MJ, Garcia-Garcia T, Ventroux M, Cherrier MV, Bazin A, Noirot P, Wilkinson AJ, Molina F, Terradot L, Noirot-Gros MF Nucleic Acids Res. 2016 Jan 8;44(1):449-63. doi: 10.1093/nar/gkv1318. Epub 2015, Nov 28. PMID:26615189<ref>PMID:26615189</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5dol" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Bacillus subtilis subsp. subtilis str. 168]]
[[Category: Large Structures]]
[[Category: Bazin A]]
[[Category: Cherrier MV]]
[[Category: Jameson KH]]
[[Category: Noirot-Gros MF]]
[[Category: Terradot L]]
[[Category: Wilkinson AJ]]