View source for 5f3j


==Crystal structure of DBP in complex with inhibitory monoclonal antibody 2D10==
<StructureSection load='5f3j' size='340' side='right'caption='[[5f3j]], [[Resolution|resolution]] 4.00&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5f3j]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus] and [https://en.wikipedia.org/wiki/Plasmodium_vivax_Sal-1 Plasmodium vivax Sal-1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5F3J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5F3J FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 4.001&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5f3j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5f3j OCA], [https://pdbe.org/5f3j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5f3j RCSB], [https://www.ebi.ac.uk/pdbsum/5f3j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5f3j ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/PVDR_PLAVS PVDR_PLAVS] Binds to the human erythrocytes Duffy blood group determinant.
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== Publication Abstract from PubMed ==
Plasmodium vivax Duffy Binding Protein (PvDBP) is the most promising vaccine candidate for P. vivax malaria. The polymorphic nature of PvDBP induces strain-specific immune responses, however, and the epitopes of broadly neutralizing antibodies are unknown. These features hamper the rational design of potent DBP-based vaccines and necessitate the identification of globally conserved epitopes. Using X-ray crystallography, small-angle X-ray scattering, hydrogen-deuterium exchange mass spectrometry, and mutational mapping, we have defined epitopes for three inhibitory mAbs (mAbs 2D10, 2H2, and 2C6) and one noninhibitory mAb (3D10) that engage DBP. These studies expand the currently known inhibitory epitope repertoire by establishing protective motifs in subdomain three outside the receptor-binding and dimerization residues of DBP, and introduce globally conserved protective targets. All of the epitopes are highly conserved among DBP alleles. The identification of broadly conserved epitopes of inhibitory antibodies provides critical motifs that should be retained in the next generation of potent vaccines for P. vivax malaria.

Broadly neutralizing epitopes in the Plasmodium vivax vaccine candidate Duffy Binding Protein.,Chen E, Salinas ND, Huang Y, Ntumngia F, Plasencia MD, Gross ML, Adams JH, Tolia NH Proc Natl Acad Sci U S A. 2016 May 18. pii: 201600488. PMID:27194724<ref>PMID:27194724</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5f3j" style="background-color:#fffaf0;"></div>

==See Also==
*[[Antibody 3D structures|Antibody 3D structures]]
*[[Erythrocyte binding antigen|Erythrocyte binding antigen]]
*[[Monoclonal Antibodies 3D structures|Monoclonal Antibodies 3D structures]]
*[[3D structures of non-human antibody|3D structures of non-human antibody]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Mus musculus]]
[[Category: Plasmodium vivax Sal-1]]
[[Category: Chen E]]
[[Category: Salinas ND]]
[[Category: Tolia NH]]