View source for 5fly


==The FhuD protein from S.pseudintermedius==
<StructureSection load='5fly' size='340' side='right'caption='[[5fly]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5fly]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_pseudintermedius Staphylococcus pseudintermedius]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FLY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5FLY FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.598&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=7PG:2,5,8,11,14,17,20,23-OCTAOXAPENTACOSAN-25-OL'>7PG</scene>, <scene name='pdbligand=CD:CADMIUM+ION'>CD</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5fly FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5fly OCA], [https://pdbe.org/5fly PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5fly RCSB], [https://www.ebi.ac.uk/pdbsum/5fly PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5fly ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A0A2P5JFB2_STAPS A0A2P5JFB2_STAPS] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Staphylococcus pseudintermedius is a leading cause of disease in dogs, and zoonosis causes human infections. Methicillin-resistant S. pseudintermedius strains are emerging, resembling the global health threat of S. aureus. Therefore, it is increasingly important to characterize potential targets for intervention against S. pseudintermedius. Here, FhuD, an S. pseudintermedius surface lipoprotein implicated in iron uptake, was characterized. It was found that FhuD bound ferrichrome in an iron-dependent manner, which increased the thermostability of FhuD by &gt;15 degrees C. The crystal structure of ferrichrome-free FhuD was determined via molecular replacement at 1.6 A resolution. FhuD exhibits the class III solute-binding protein (SBP) fold, with a ligand-binding cavity between the N- and C-terminal lobes, which is here occupied by a PEG molecule. The two lobes of FhuD were oriented in a closed conformation. These results provide the first detailed structural characterization of FhuD, a potential therapeutic target of S. pseudintermedius.

Crystal structure of FhuD at 1.6 A resolution: a ferrichrome-binding protein from the animal and human pathogen Staphylococcus pseudintermedius.,Abate F, Cozzi R, Maritan M, Lo Surdo P, Maione D, Malito E, Bottomley MJ Acta Crystallogr F Struct Biol Commun. 2016 Mar 1;72(Pt 3):214-9. doi:, 10.1107/S2053230X16002272. Epub 2016 Feb 19. PMID:26919525<ref>PMID:26919525</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5fly" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Staphylococcus pseudintermedius]]
[[Category: Malito E]]