View source for 5flz


==Cryo-EM structure of gamma-TuSC oligomers in a closed conformation==
<SX load='5flz' size='340' side='right' viewer='molstar' caption='[[5flz]], [[Resolution|resolution]] 6.90&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5flz]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FLZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5FLZ FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 6.9&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5flz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5flz OCA], [https://pdbe.org/5flz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5flz RCSB], [https://www.ebi.ac.uk/pdbsum/5flz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5flz ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/SPC97_YEAST SPC97_YEAST] Involved in microtubule organization by the microtubule organizing center, the spindle pole body (SPB). Probably part of the microtubule attachment site at the SPB.
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== Publication Abstract from PubMed ==
Modeling protein complex structures based on distantly related homologues can be challenging due to poor sequence and structure conservation. Therefore, utilizing even low-resolution experimental data can significantly increase model precision and accuracy. Here, we present models of the two key functional states of the yeast gamma-tubulin small complex (gammaTuSC): one for the low-activity "open" state and another for the higher-activity "closed" state. Both models were computed based on remotely related template structures and cryo-EM density maps at 6.9A and 8.0A resolution, respectively. For each state, extensive sampling of alignments and conformations was guided by the fit to the corresponding cryo-EM density map. The resulting good-scoring models formed a tightly clustered ensemble of conformations in most regions. We found significant structural differences between the two states, primarily in the gamma-tubulin subunit regions where the microtubule binds. We also report a set of chemical cross-links that were found to be consistent with equilibrium between the open and closed states. The protocols developed here have been incorporated into our open-source Integrative Modeling Platform (IMP) software package (http://integrativemodeling.org), and can therefore be applied to many other systems.

Structure of gamma-tubulin small complex based on a cryo-EM map, chemical cross-links, and a remotely related structure.,Greenberg CH, Kollman J, Zelter A, Johnson R, MacCoss MJ, Davis TN, Agard DA, Sali A J Struct Biol. 2016 Jun;194(3):303-10. doi: 10.1016/j.jsb.2016.03.006. Epub 2016 , Mar 8. PMID:26968363<ref>PMID:26968363</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 5flz" style="background-color:#fffaf0;"></div>

==See Also==
*[[Tubulin 3D Structures|Tubulin 3D Structures]]
== References ==
<references/>
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</SX>
[[Category: Large Structures]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Agard DA]]
[[Category: Davis TN]]
[[Category: Greenberg CH]]
[[Category: Johnson R]]
[[Category: Kollman J]]
[[Category: MacCoss MJ]]
[[Category: Sali A]]
[[Category: Zelter A]]