View source for 5foz


==De novo structure of the binary mosquito larvicide BinAB at pH 10==
<StructureSection load='5foz' size='340' side='right'caption='[[5foz]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5foz]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Lysinibacillus_sphaericus Lysinibacillus sphaericus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FOZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5FOZ FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5foz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5foz OCA], [https://pdbe.org/5foz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5foz RCSB], [https://www.ebi.ac.uk/pdbsum/5foz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5foz ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/BINA1_LYSSH BINA1_LYSSH] This toxin is active against Culex and Anopheles.
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
BinAB is a naturally occurring paracrystalline larvicide distributed worldwide to combat the devastating diseases borne by mosquitoes. These crystals are composed of homologous molecules, BinA and BinB, which play distinct roles in the multi-step intoxication process, transforming from harmless, robust crystals, to soluble protoxin heterodimers, to internalized mature toxin, and finally to toxic oligomeric pores. The small size of the crystals-50 unit cells per edge, on average-has impeded structural characterization by conventional means. Here we report the structure of Lysinibacillus sphaericus BinAB solved de novo by serial-femtosecond crystallography at an X-ray free-electron laser. The structure reveals tyrosine- and carboxylate-mediated contacts acting as pH switches to release soluble protoxin in the alkaline larval midgut. An enormous heterodimeric interface appears to be responsible for anchoring BinA to receptor-bound BinB for co-internalization. Remarkably, this interface is largely composed of propeptides, suggesting that proteolytic maturation would trigger dissociation of the heterodimer and progression to pore formation.

De novo phasing with X-ray laser reveals mosquito larvicide BinAB structure.,Colletier JP, Sawaya MR, Gingery M, Rodriguez JA, Cascio D, Brewster AS, Michels-Clark T, Hice RH, Coquelle N, Boutet S, Williams GJ, Messerschmidt M, DePonte DP, Sierra RG, Laksmono H, Koglin JE, Hunter MS, Park HW, Uervirojnangkoorn M, Bideshi DK, Brunger AT, Federici BA, Sauter NK, Eisenberg DS Nature. 2016 Sep 28. doi: 10.1038/nature19825. PMID:27680699<ref>PMID:27680699</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5foz" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Lysinibacillus sphaericus]]
[[Category: Bideshi DL]]
[[Category: Boutet S]]
[[Category: Brewster AS]]
[[Category: Brunger AT]]
[[Category: Cascio D]]
[[Category: Colletier JP]]
[[Category: DePonte DP]]
[[Category: Eisenberg DS]]
[[Category: Federici BA]]
[[Category: Gingery M]]
[[Category: Hunter MS]]
[[Category: Koglin JE]]
[[Category: Laksmono H]]
[[Category: Messerschmidt M]]
[[Category: Michels-Clark T]]
[[Category: Rodriguez JA]]
[[Category: Sauter NK]]
[[Category: Sawaya MR]]
[[Category: Sierra RG]]
[[Category: Uervirojnangkoorn M]]
[[Category: W Park H]]
[[Category: Williams GJ]]