View source for 5frr


==Structure of the Pds5-Scc1 complex and implications for cohesin function==
<StructureSection load='5frr' size='340' side='right'caption='[[5frr]], [[Resolution|resolution]] 5.80&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5frr]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FRR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5FRR FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 5.8&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5frr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5frr OCA], [https://pdbe.org/5frr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5frr RCSB], [https://www.ebi.ac.uk/pdbsum/5frr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5frr ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/PDS5_YEAST PDS5_YEAST] Essential for the establishment and maintenance of sister chromatid cohesion at centromere proximal and distal regions during S phase. Also required for chromosomal condensation.<ref>PMID:11062262</ref> <ref>PMID:11137006</ref> 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Sister chromatid cohesion is a fundamental prerequisite to faithful genome segregation. Cohesion is precisely regulated by accessory factors that modulate the stability with which the cohesin complex embraces chromosomes. One of these factors, Pds5, engages cohesin through Scc1 and is both a facilitator of cohesion, and, conversely also mediates the release of cohesin from chromatin. We present here the crystal structure of a complex between budding yeast Pds5 and Scc1, thus elucidating the molecular basis of Pds5 function. Pds5 forms an elongated HEAT repeat that binds to Scc1 via a conserved surface patch. We demonstrate that the integrity of the Pds5-Scc1 interface is indispensable for the recruitment of Pds5 to cohesin, and that its abrogation results in loss of sister chromatid cohesion and cell viability.

Structure of the Pds5-Scc1 Complex and Implications for Cohesin Function.,Muir KW, Kschonsak M, Li Y, Metz J, Haering CH, Panne D Cell Rep. 2016 Feb 24. pii: S2211-1247(16)30094-8. doi:, 10.1016/j.celrep.2016.01.078. PMID:26923589<ref>PMID:26923589</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5frr" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Haering CH]]
[[Category: Kschonsak M]]
[[Category: Li Y]]
[[Category: Metz J]]
[[Category: Muir KW]]
[[Category: Panne D]]