View source for 5fsw


==RNA dependent RNA polymerase QDE-1 from Thielavia terrestris==
<StructureSection load='5fsw' size='340' side='right'caption='[[5fsw]], [[Resolution|resolution]] 3.19&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5fsw]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermothielavioides_terrestris Thermothielavioides terrestris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FSW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5FSW FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.19&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5fsw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5fsw OCA], [https://pdbe.org/5fsw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5fsw RCSB], [https://www.ebi.ac.uk/pdbsum/5fsw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5fsw ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/G2R911_THETT G2R911_THETT] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Many eukaryotic organisms encode more than one RNA-dependent RNA polymerase (RdRP) that likely emerged as a result of gene duplication. Such RdRP paralogs often participate in distinct RNA silencing pathways and show characteristic repertoires of enzymatic activities in vitro. However, to what extent members of individual paralogous groups can undergo functional changes during speciation remains an open question. We show that orthologs of QDE-1, an RdRP component of the quelling pathway in Neurospora crassa, have rapidly diverged in evolution at the amino acid sequence level. Analyses of purified QDE-1 polymerases from N. crassa (QDE-1Ncr) and related fungi, Thielavia terrestris (QDE-1Tte) and Myceliophthora thermophila (QDE-1Mth), show that all three enzymes can synthesize RNA but the precise modes of their action differ considerably. Unlike their QDE-1Ncr counterpart favoring processive RNA synthesis, QDE-1Tte and QDE-1Mth produce predominantly short RNA copies via primer-independent initiation. Surprisingly, a 3.19 A-resolution crystal structure of QDE-1Tte reveals a quasi-symmetric dimer similar to QDE-1Ncr. Further electron microscopy analyses confirm that QDE-1Tte occurs as a dimer in solution and retains this status upon interaction with a template. We conclude that divergence of orthologous RdRPs can result in functional innovation while retaining overall protein fold and quaternary structure.

Functional Evolution in Orthologous Cell-encoded RNA-dependent RNA Polymerases.,Qian X, Hamid FM, El Sahili A, Darwis DA, Wong YH, Bhushan S, Makeyev EV, Lescar J J Biol Chem. 2016 Feb 23. pii: jbc.M115.685933. PMID:26907693<ref>PMID:26907693</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5fsw" style="background-color:#fffaf0;"></div>

==See Also==
*[[RNA polymerase 3D structures|RNA polymerase 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Thermothielavioides terrestris]]
[[Category: Bhushan S]]
[[Category: Darwis DA]]
[[Category: El Sahili A]]
[[Category: Hamid FM]]
[[Category: Lescar J]]
[[Category: Makeyev EV]]
[[Category: Qian X]]
[[Category: Wong YH]]