View source for 5ftz


==AA10 lytic polysaccharide monooxygenase (LPMO) from Streptomyces lividans==
<StructureSection load='5ftz' size='340' side='right'caption='[[5ftz]], [[Resolution|resolution]] 1.38&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5ftz]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_lividans_1326 Streptomyces lividans 1326]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FTZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5FTZ FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.38&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5ftz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ftz OCA], [https://pdbe.org/5ftz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5ftz RCSB], [https://www.ebi.ac.uk/pdbsum/5ftz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5ftz ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/D6EWM4_STRLI D6EWM4_STRLI] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Copper-dependent lytic polysaccharide monooxygenases (LPMOs) are enzymes that oxidatively deconstruct polysaccharides. The active site copper in LPMOs is coordinated by a histidine-brace. This utilises the amino group and side chain of the N-terminal His residue with the side chain of a second His residue to create a T-shaped arrangement of nitrogen ligands. We report a structural, kinetic and thermodynamic appraisal of copper binding to the histidine-brace in an auxiliary activity family 10 (AA10) LPMO from Streptomyces lividans (SliLPMO10E). Unexpectedly we discover the existence of two apo-SliLPMO10E species in solution that can each bind copper at a single site with distinct kinetic and thermodynamic (exothermic and endothermic) properties. The experimental EPR spectrum of copper-bound SliLPMO10E requires the simulation of two different line shapes, implying two different copper bound species, indicative of three and two nitrogen ligands coordinating the copper. Amino group coordination was probed through the creation of an N-terminal extension variant (SliLPMO10E-Ext). The kinetics and thermodynamics of copper binding to SliLPMO10E-Ext are in accord with copper binding to one of the apo-forms in the wild-type protein, suggesting that amino group coordination is absent in the two nitrogen coordinate form of SliLPMO10E. Copper binding to SliLPMO10B was also investigated, and again revealed the presence of two apo-forms with kinetics and stoichiometry of copper binding identical to that of SliLPMO10E. Our findings highlight that heterogeneity exists in the active site copper coordination sphere of LPMOs that may have implications for the mechanism of loading copper in the cell.

Heterogeneity in the histidine-brace copper coordination sphere in AA10 lytic polysaccharide monooxygenases.,Chaplin AK, Wilson MT, Hough MA, Svistunenko DA, Hemsworth GR, Walton PH, Vijgenboom E, Worrall JA J Biol Chem. 2016 Apr 15. pii: jbc.M116.722447. PMID:27129229<ref>PMID:27129229</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5ftz" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Streptomyces lividans 1326]]
[[Category: Chaplin AKC]]
[[Category: Hemsworth GR]]
[[Category: Hough MA]]
[[Category: Svistunenko DA]]
[[Category: Vijgenboom E]]
[[Category: Walton PH]]
[[Category: Wilson MT]]
[[Category: Worrall JAR]]