View source for 5fui


==Crystal structure of the C-terminal CBM6 of LamC a marine laminarianse from Zobellia galactanivorans==
<StructureSection load='5fui' size='340' side='right'caption='[[5fui]], [[Resolution|resolution]] 1.40&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5fui]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Zobellia_galactanivorans Zobellia galactanivorans]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=4crr 4crr]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FUI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5FUI FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.4&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=APY:2-AMINOMETHYL-PYRIDINE'>APY</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5fui FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5fui OCA], [https://pdbe.org/5fui PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5fui RCSB], [https://www.ebi.ac.uk/pdbsum/5fui PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5fui ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/G0L2L9_ZOBGA G0L2L9_ZOBGA] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Laminarin is an abundant brown algal storage polysaccharide. Marine microorganisms, such as Zobellia galactanivorans, produce laminarinases for its degradation, which are important for the processing of this organic matter in the ocean carbon cycle. These laminarinases are often modular, as is the case with ZgLamC which has an N-terminal GH16 module, a central family 6 carbohydrate-binding module (CBM) and a C-terminal PorSS module. To date no studies have characterized a true marine laminarin binding CBM6 with its natural carbohydrate ligand. The crystal structure of ZgLamCCBM 6 indicates that this CBM has two clefts for binding sugar (Variable loop site, VLS, and concave face site, CFS). The ZgLamCCBM 6 VLS binds in an exo-manner and the CFS interacts in an endo manner with laminarin. Isothermal titration calorimetry experiments on native and mutant ZgLamCCBM 6 confirm that these binding sites have different modes of recognition for laminarin, in agreement with the 'regional model' postulated for CBM6 binding modules. Based on isothermal titration calorimetry data and structural data we propose a model of ZgLamCCBM 6 interacting with different chains of laminarin in a multivalent manner, forming a complex cross-linked protein-polysaccharide network. This article is protected by copyright. All rights reserved.

Unraveling the multivalent binding of a marine family 6 carbohydrate-binding module with its native laminarin ligand.,Jam M, Ficko-Blean E, Labourel A, Larocque R, Czjzek M, Michel G FEBS J. 2016 Mar 9. doi: 10.1111/febs.13707. PMID:26959085<ref>PMID:26959085</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5fui" style="background-color:#fffaf0;"></div>

==See Also==
*[[Glucanase 3D structures|Glucanase 3D structures]]
*[[Laminarase 3D structures|Laminarase 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Zobellia galactanivorans]]
[[Category: Czjzek M]]
[[Category: Ferrieres V]]
[[Category: Ficko-Blean E]]
[[Category: Hehemann JH]]
[[Category: Jam M]]
[[Category: Labourel A]]
[[Category: Legentil L]]
[[Category: Michel G]]
[[Category: Sylla B]]