View source for 5fv6


==KpFlo11 presents a novel member of the Flo11 family with a unique recognition pattern for homophilic interactions==
<StructureSection load='5fv6' size='340' side='right'caption='[[5fv6]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5fv6]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Komagataella_phaffii_GS115 Komagataella phaffii GS115]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FV6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5FV6 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5fv6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5fv6 OCA], [https://pdbe.org/5fv6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5fv6 RCSB], [https://www.ebi.ac.uk/pdbsum/5fv6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5fv6 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/FLO11_KOMPG FLO11_KOMPG] Homophilic binding protein that enables kin discrimination in heterogeneous yeast populations by mediating homotypic cell-cell interactions during flocculation, a reversible and asexual process in which cells adhere to form aggregates (flocs).<ref>PMID:32286952</ref> 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Microorganisms have evolved specific cell surface molecules that enable discrimination between cells from the same and from a different kind. Here, we investigate the role of Flo11-type cell surface adhesins from social yeasts in kin discrimination. We measure the adhesion forces mediated by Flo11A-type domains using single-cell force spectroscopy, quantify Flo11A-based cell aggregation in populations and determine the Flo11A-dependent segregation of competing yeast strains in biofilms. We find that Flo11A domains from diverse yeast species confer remarkably strong adhesion forces by establishing homotypic interactions between single cells, leading to efficient cell aggregation and biofilm formation in homogenous populations. Heterotypic interactions between Flo11A domains from different yeast species or Saccharomyces cerevisiae strains confer weak adhesive forces and lead to efficient strain segregation in heterogenous populations, indicating that in social yeasts Flo11A-mediated cell adhesion is a major mechanism for kin discrimination at species and sub-species levels. These findings, together with our structure and mutation analysis of selected Flo11A domains, provide a rationale of how cell surface receptors have evolved in microorganisms to mediate kin discrimination.

Kin discrimination in social yeast is mediated by cell surface receptors of the Flo11 adhesin family.,Bruckner S, Schubert R, Kraushaar T, Hartmann R, Hoffmann D, Jelli E, Drescher K, Muller DJ, Oliver Essen L, Mosch HU Elife. 2020 Apr 14;9. pii: 55587. doi: 10.7554/eLife.55587. PMID:32286952<ref>PMID:32286952</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5fv6" style="background-color:#fffaf0;"></div>

==See Also==
*[[Flocculation protein 3D structures|Flocculation protein 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Komagataella phaffii GS115]]
[[Category: Large Structures]]
[[Category: Brueckner S]]
[[Category: Essen LO]]
[[Category: Kraushaar T]]
[[Category: Mikolaiski M]]
[[Category: Moesch HU]]
[[Category: Schreiner F]]
[[Category: Veelders M]]