View source for 5fwo


==Llama nanobody PorM_130==
<StructureSection load='5fwo' size='340' side='right'caption='[[5fwo]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5fwo]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Lama_glama Lama glama]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FWO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5FWO FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5fwo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5fwo OCA], [https://pdbe.org/5fwo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5fwo RCSB], [https://www.ebi.ac.uk/pdbsum/5fwo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5fwo ProSAT]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
PorM is a membrane protein that is involved in the assembly of the type IX secretion system (T9SS) in Porphyromonas gingivalis, a major bacterial pathogen that is responsible for periodontal disease in humans. In the context of structural studies of PorM to better understand T9SS assembly, four camelid nanobodies were selected, produced and purified, and their specific interaction with the N-terminal or C-terminal part of the periplasmic domain of PorM was investigated. Diffracting crystals were also obtained, and the structures of the four nanobodies were solved by molecular replacement. Furthermore, two nanobodies were used as crystallization chaperones and turned out to be valuable tools in the structure-determination process of the periplasmic domain of PorM.

Camelid nanobodies used as crystallization chaperones for different constructs of PorM, a component of the type IX secretion system from Porphyromonas gingivalis.,Duhoo Y, Roche J, Trinh TTN, Desmyter A, Gaubert A, Kellenberger C, Cambillau C, Roussel A, Leone P Acta Crystallogr F Struct Biol Commun. 2017 May 1;73(Pt 5):286-293. doi:, 10.1107/S2053230X17005969. Epub 2017 Apr 26. PMID:28471361<ref>PMID:28471361</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
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== References ==
<references/>
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</StructureSection>
[[Category: Lama glama]]
[[Category: Large Structures]]
[[Category: Leone P]]
[[Category: Roussel A]]