View source for 5g35


==Structure of Rad14 in complex with acetylaminopyren-C8-guanine containing DNA==
<StructureSection load='5g35' size='340' side='right'caption='[[5g35]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5g35]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5G35 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5G35 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=8PY:[(2~{R},3~{S},5~{R})-5-[2-AZANYL-8-[ETHANOYL(PYREN-2-YL)AMINO]-6-OXIDANYLIDENE-1~{H}-PURIN-9-YL]-3-OXIDANYL-OXOLAN-2-YL]METHYL+DIHYDROGEN+PHOSPHATE'>8PY</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5g35 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5g35 OCA], [https://pdbe.org/5g35 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5g35 RCSB], [https://www.ebi.ac.uk/pdbsum/5g35 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5g35 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/RAD14_YEAST RAD14_YEAST] Involved in nucleotide excision repair. Binds specifically to damaged DNA. Required for the incision step.
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Heterocyclic aromatic amines react with purine bases and result in bulky DNA adducts which cause mutations. Such structurally diverse lesions are substrates for the nucleotide excision repair (NER). It is thought that the NER machinery recognises distorted DNA conformations, involving the xeroderma pigmentosum group A and C (XPA, XPC) proteins as a scaffold between the DNA substrate and several other NER proteins. Here we present the synthesis of DNA containing the polycyclic aromatic amine C8-guanine lesions acetylaminophenyl, acetylaminonaphthyl, acetylaminoanthryl and acetylaminopyrenyl as well as their crystal structures in in complex with the yeast XPA homologue Rad14. This work further substantiates the indirect lesion detection mechanism employed by the NER system to recognise destabilised and deformable DNA structures.

Structural basis for bulky adduct DNA lesion recognition by the nucleotide excision repair protein Rad14.,Schneider S, Simon N, Ebert C Chemistry. 2016 May 25. doi: 10.1002/chem.201602438. PMID:27223336<ref>PMID:27223336</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5g35" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Synthetic construct]]
[[Category: Ebert C]]
[[Category: Schneider S]]
[[Category: Simon N]]