View source for 5g62


==S.pneumoniae ABC-transporter substrate binding protein FusA EF-hand mutant in complex with fructo-nystose==
<StructureSection load='5g62' size='340' side='right'caption='[[5g62]], [[Resolution|resolution]] 1.99&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5g62]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_pneumoniae_TIGR4 Streptococcus pneumoniae TIGR4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5G62 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5G62 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.99&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=FRU:FRUCTOSE'>FRU</scene>, <scene name='pdbligand=PRD_900056:1-FRUCTOFURANOSYL-D-NYSTOSE'>PRD_900056</scene>, <scene name='pdbligand=UEA:beta-D-sorbofuranose'>UEA</scene>, <scene name='pdbligand=ZEE:beta-L-talopyranose'>ZEE</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5g62 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5g62 OCA], [https://pdbe.org/5g62 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5g62 RCSB], [https://www.ebi.ac.uk/pdbsum/5g62 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5g62 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/FUSA_STRPN FUSA_STRPN] Part of the ABC transporter complex FusABC-MsmK involved in short- and long-chain fructooligosaccharide (FOS) import. Required for the utilization of long-chain FOSs (PubMed:23264576). Binds kestose, nystose, fructofuranosyl-nystose and inulin, but not sucrose. Has a preference for long-chain FOSs (tetrasaccharides and larger) (PubMed:27939783).<ref>PMID:27939783</ref> <ref>PMID:23264576</ref> 
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== Publication Abstract from PubMed ==
Streptococcus pneumoniae is dependent on carbohydrate uptake for colonization and pathogenesis, and dedicates over a third of its transport systems to their uptake. The ability of the pneumococcus to utilize fructooligosaccharides (FOSs) is attributed to the presence of one of two types of FOS ATP-binding cassette (ABC) transporters. Strains encoding SfuABC are only able to utilize short-chain FOSs, while strains encoding FusABC can utilize both short- and long-chain FOSs. The crystal structures of the substrate-binding protein FusA in its open and closed conformations bound to FOSs, and solution scattering data of SfuA, delineate the structural basis for import of short- and long-chain FOSs. The structure of FusA identifies an EF hand-like calcium-binding motif. This is shown to be essential for translocation of FOSs in FusABC and forms the basis for the definition of a new class of substrate-binding proteins that regulate substrate translocation by calcium.

Structural Basis for Regulation and Specificity of Fructooligosaccharide Import in Streptococcus pneumoniae.,Culurgioni S, Harris G, Singh AK, King SJ, Walsh MA Structure. 2017 Jan 3;25(1):79-93. doi: 10.1016/j.str.2016.11.008. Epub 2016 Dec , 8. PMID:27939783<ref>PMID:27939783</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 5g62" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Streptococcus pneumoniae TIGR4]]
[[Category: Culurgioni S]]
[[Category: Harris G]]
[[Category: King SJ]]
[[Category: Singh AK]]
[[Category: Walsh MA]]