View source for 5gjr


==An atomic structure of the human 26S proteasome==
<SX load='5gjr' size='340' side='right' viewer='molstar' caption='[[5gjr]], [[Resolution|resolution]] 3.50&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5gjr]] is a 20 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5GJR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5GJR FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.5&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5gjr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5gjr OCA], [https://pdbe.org/5gjr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5gjr RCSB], [https://www.ebi.ac.uk/pdbsum/5gjr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5gjr ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/PSB5_HUMAN PSB5_HUMAN] The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This unit is responsible of the chymotrypsin-like activity of the proteasome and is one of the principal target of the proteasome inhibitor bortezomib. May catalyze basal processing of intracellular antigens. Plays a role in the protection against oxidative damage through the Nrf2-ARE pathway (By similarity).
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
We report the cryo-EM structure of the human 26S proteasome at an average resolution of 3.5 A, allowing atomic modeling of 28 subunits in the core particle (CP) and 18 subunits in the regulatory particle (RP). The C-terminal residues of Rpt3 and Rpt5 subunits in the RP can be seen inserted into surface pockets formed between adjacent alpha subunits in the CP. Each of the six Rpt subunits contains a bound nucleotide, and the central gate of the CP alpha-ring is closed despite RP association. The six pore 1 loops in the Rpt ring are arranged similarly to a spiral staircase along the axial channel of substrate transport, which is constricted by the pore 2 loops. We also determined the cryo-EM structure of the human proteasome bound to the deubiquitinating enzyme USP14 at 4.35-A resolution. Together, our structures provide a framework for mechanistic understanding of eukaryotic proteasome function.

An atomic structure of the human 26S proteasome.,Huang X, Luan B, Wu J, Shi Y Nat Struct Mol Biol. 2016 Jul 18. doi: 10.1038/nsmb.3273. PMID:27428775<ref>PMID:27428775</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5gjr" style="background-color:#fffaf0;"></div>

==See Also==
*[[Proteasome 3D structures|Proteasome 3D structures]]
== References ==
<references/>
__TOC__
</SX>
[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Huang XL]]
[[Category: Luan B]]
[[Category: Shi YG]]
[[Category: Wu JP]]