View source for 5gtx


==Crystal structure of mutated buckwheat glutaredoxin==
<StructureSection load='5gtx' size='340' side='right'caption='[[5gtx]], [[Resolution|resolution]] 2.28&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5gtx]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Polygonaceae Polygonaceae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5GTX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5GTX FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.28&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5gtx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5gtx OCA], [https://pdbe.org/5gtx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5gtx RCSB], [https://www.ebi.ac.uk/pdbsum/5gtx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5gtx ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A0A1W6I4R7_FAGTA A0A1W6I4R7_FAGTA] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Glutaredoxins (Grxs) are ubiquitous thioltransferases and members of the thioredoxin (Trx) fold superfamily. They have multiple functions in cells including oxidative stress responses and cell signaling. A novel glutaredoxin from buckwheat (rbGrx) with higher catalytic activity was identified, cloned, and purified. The structures of glutathionylated rbGrx and an rbGrx mutant, in which cysteine 39 was mutated to alanine, were solved by x-ray diffraction at a resolution of 2.05A and 2.29A, respectively. In rbGrx, GSH (glutathione) is bound at the conserved GSH-binding site, and its structure shows that it has the potential to function as a scaffold protein for the assembly and delivery of GSH. The crystal structure shows that GSH does not bind to the C39A rbGrx mutant, and the C39A mutant had no catalytic activity, indicating that C39 is a key residue that is involved in both the binding of rbGrx to GSH and the regulation of its catalytic activity. The model showing the binding of GSH with rbGrx provides a basis for understanding its molecular function and its potential future applications in medicinal food science.

Structural insights into the binding of buckwheat glutaredoxin with GSH and regulation of its catalytic activity.,Zhang X, Wang W, Li C, Zhao Y, Yuan H, Tan X, Wu L, Wang Z, Wang H J Inorg Biochem. 2017 Aug;173:21-27. doi: 10.1016/j.jinorgbio.2017.04.019. Epub, 2017 Apr 27. PMID:28478310<ref>PMID:28478310</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5gtx" style="background-color:#fffaf0;"></div>
== References ==
<references/>
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</StructureSection>
[[Category: Large Structures]]
[[Category: Polygonaceae]]
[[Category: Wang H]]
[[Category: Wang W]]
[[Category: Wang Z]]
[[Category: Zhang X]]
[[Category: Zhao Y]]