View source for 5gx6


==Crystal structure of solute-binding protein complexed with unsaturated chondroitin disaccharide with a sulfate group at C-4 position of GalNAc==
<StructureSection load='5gx6' size='340' side='right'caption='[[5gx6]], [[Resolution|resolution]] 1.81&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5gx6]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptobacillus_moniliformis_DSM_12112 Streptobacillus moniliformis DSM 12112]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5GX6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5GX6 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.81&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ASG:2-DEOXY-2-ACETAMIDO-BETA-D-GALACTOSE-4-SULFATE'>ASG</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GCD:4,5-DEHYDRO-D-GLUCURONIC+ACID'>GCD</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5gx6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5gx6 OCA], [https://pdbe.org/5gx6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5gx6 RCSB], [https://www.ebi.ac.uk/pdbsum/5gx6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5gx6 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/D1AWE0_STRM9 D1AWE0_STRM9] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Glycosaminoglycans (GAGs), such as hyaluronan, chondroitin sulfate, and heparin, constitute mammalian extracellular matrices. The uronate and amino sugar residues in hyaluronan and chondroitin sulfate are linked by 1,3-glycoside bond, while heparin contains 1,4-glycoside bond. Some bacteria target GAGs as means of establishing colonization and/or infection, and bacterial degradation mechanisms of GAGs have been well characterized. However, little is known about the bacterial import of GAGs. Here, we show a GAG import system, comprised of a solute-binding protein (Smon0123)-dependent ATP-binding cassette (ABC) transporter, in the pathogenic Streptobacillus moniliformis. A genetic cluster responsible for depolymerization, degradation, and metabolism of GAGs as well as the ABC transporter system was found in the S. moniliformis genome. This bacterium degraded hyaluronan and chondroitin sulfate with an expression of the genetic cluster, while heparin repressed the bacterial growth. The purified recombinant Smon0123 exhibited an affinity with disaccharides generated from hyaluronan and chondroitin sulfate. X-ray crystallography indicated binding mode of Smon0123 to GAG disaccharides. The purified recombinant ABC transporter as a tetramer (Smon0121-Smon0122/Smon0120-Smon0120) reconstructed in liposomes enhanced its ATPase activity in the presence of Smon0123 and GAG disaccharides. This is the first report that has molecularly depicted a bacterial import system of both sulfated and non-sulfated GAGs.

A bacterial ABC transporter enables import of mammalian host glycosaminoglycans.,Oiki S, Mikami B, Maruyama Y, Murata K, Hashimoto W Sci Rep. 2017 Apr 21;7(1):1069. doi: 10.1038/s41598-017-00917-y. PMID:28432302<ref>PMID:28432302</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5gx6" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Streptobacillus moniliformis DSM 12112]]
[[Category: Hashimoto W]]
[[Category: Mikami B]]
[[Category: Murata K]]
[[Category: Oiki S]]