View source for 5gzk


==Endo-beta-1,2-glucanase from Chitinophaga pinensis - sophorotriose and glucose complex==
<StructureSection load='5gzk' size='340' side='right'caption='[[5gzk]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5gzk]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Chitinophaga_pinensis_DSM_2588 Chitinophaga pinensis DSM 2588]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5GZK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5GZK FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=1PE:PENTAETHYLENE+GLYCOL'>1PE</scene>, <scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5gzk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5gzk OCA], [https://pdbe.org/5gzk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5gzk RCSB], [https://www.ebi.ac.uk/pdbsum/5gzk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5gzk ProSAT]</span></td></tr>
</table>
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== Publication Abstract from PubMed ==
beta-1,2-Glucan is an extracellular cyclic or linear polysaccharide from Gram-negative bacteria, with important roles in infection and symbiosis. Despite beta-1,2-glucan's importance in bacterial persistence and pathogenesis, only a few reports exist on enzymes acting on both cyclic and linear beta-1,2-glucan. To this end, we purified an endo-beta-1,2-glucanase to homogeneity from cell extracts of the environmental species Chitinophaga arvensicola, and an endo-beta-1,2-glucanase candidate gene (Cpin_6279) was cloned from the related species Chitinophaga pinensis The Cpin_6279 protein specifically hydrolyzed linear beta-1,2-glucan with polymerization degrees of &gt;/=5 and a cyclic counterpart, indicating that Cpin_6279 is an endo-beta-1,2-glucananase. Stereochemical analysis demonstrated that the Cpin_6279-catalyzed reaction proceeds via an inverting mechanism. Cpin_6279 exhibited no significant sequence similarity with known glycoside hydrolases (GHs), and thus the enzyme defines a novel GH family, GH144. The crystal structures of the ligand-free and complex forms of Cpin_6279 with glucose (Glc) and sophorotriose (Glc-beta-1,2-Glc-beta-1,2-Glc) determined up to 1.7 A revealed that it has a large cavity appropriate for polysaccharide degradation and adopts an (alpha/alpha)6-fold slightly similar to that of GH family 15 and 8 enzymes. Mutational analysis indicated that some of the highly conserved acidic residues in the active site are important for catalysis, and the Cpin_6279 active-site architecture provided insights into the substrate recognition by the enzyme. The biochemical characterization and crystal structure of this novel GH may enable discovery of other beta-1,2-glucanases and represent a critical advance toward elucidating structure-function relationships of GH enzymes.

Biochemical and structural analyses of a bacterial endo-beta-1,2-glucanase reveal a new glycoside hydrolase family.,Abe K, Nakajima M, Yamashita T, Matsunaga H, Kamisuki S, Nihira T, Takahashi Y, Sugimoto N, Miyanaga A, Nakai H, Arakawa T, Fushinobu S, Taguchi H J Biol Chem. 2017 May 5;292(18):7487-7506. doi: 10.1074/jbc.M116.762724. Epub, 2017 Mar 7. PMID:28270506<ref>PMID:28270506</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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== References ==
<references/>
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</StructureSection>
[[Category: Chitinophaga pinensis DSM 2588]]
[[Category: Large Structures]]
[[Category: Abe K]]
[[Category: Arakawa T]]
[[Category: Fushinobu S]]
[[Category: Nakajima M]]
[[Category: Taguchi H]]