View source for 5h20


==X-ray structure of PadR-like Transcription factor from bacteroid fragilis==
<StructureSection load='5h20' size='340' side='right'caption='[[5h20]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5h20]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacteroides_fragilis_NCTC_9343 Bacteroides fragilis NCTC 9343]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5H20 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5H20 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=IPA:ISOPROPYL+ALCOHOL'>IPA</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5h20 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5h20 OCA], [https://pdbe.org/5h20 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5h20 RCSB], [https://www.ebi.ac.uk/pdbsum/5h20 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5h20 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q5LC36_BACFN Q5LC36_BACFN] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
A phenolic acid decarboxylase (padC) regulator, PadR and its homologs proteins belong to the PadR family. Despite the growing numbers of the PadR family members and their various roles in bacteria, such as detoxifications, drug transports and circadian rhythms, biochemical and biophysical studies of the PadR family are very limited. Thus, a ligand-induced regulatory mechanism of the PadR family transcription factors remains to be elucidated. Here, we report a crystal structure of a Bacteroides fragilis PadR-like protein, BF2549 and revealed its interaction with putative operator DNA and ligand molecules. Comparative structural and primary sequence analyses provide a PadR-specific motif that is conserved in the PadR family but deviated from the MarR family. Furthermore, putative ligand binding sites are observed in the BF2549 structure. Finally, a homology-based structure model of BF2549 and 29-mer dsDNA propose regulatory mechanisms of the PadR family in transcriptional derepression.

Structural and functional analysis of BF2549, a PadR-like transcription factor from Bacteroides fragilis.,Lee C, Kim MI, Hong M Biochem Biophys Res Commun. 2017 Jan 29;483(1):264-270. doi:, 10.1016/j.bbrc.2016.12.155. Epub 2016 Dec 24. PMID:28027933<ref>PMID:28027933</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5h20" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Bacteroides fragilis NCTC 9343]]
[[Category: Large Structures]]
[[Category: Hong M]]
[[Category: Lee C]]