View source for 5h81


==HETEROYOHIMBINE SYNTHASE THAS2 FROM CATHARANTHUS ROSEUS - COMPLEX WITH NADP+==
<StructureSection load='5h81' size='340' side='right'caption='[[5h81]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5h81]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Catharanthus_roseus Catharanthus roseus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5H81 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5H81 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5h81 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5h81 OCA], [https://pdbe.org/5h81 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5h81 RCSB], [https://www.ebi.ac.uk/pdbsum/5h81 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5h81 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A0A1C7D193_CATRO A0A1C7D193_CATRO] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Plants produce an enormous array of biologically active metabolites, often with stereochemical variations on the same molecular scaffold. These changes in stereochemistry dramatically impact biological activity. Notably, the stereoisomers of the heteroyohimbine alkaloids show diverse pharmacological activities. We reported a medium chain dehydrogenase/reductase (MDR) from Catharanthus roseus that catalyses formation of a heteroyohimbine isomer. Here we report the discovery of additional heteroyohimbine synthases (HYSs), one of which produces a mixture of diastereomers. The crystal structures for three HYSs have been solved, providing insight into the mechanism of reactivity and stereoselectivity, with mutation of one loop transforming product specificity. Localization and gene silencing experiments provide a basis for understanding the function of these enzymes in vivo. This work sets the stage to explore how MDRs evolved to generate structural and biological diversity in specialized plant metabolism and opens the possibility for metabolic engineering of new compounds based on this scaffold.

Structural investigation of heteroyohimbine alkaloid synthesis reveals active site elements that control stereoselectivity.,Stavrinides A, Tatsis EC, Caputi L, Foureau E, Stevenson CE, Lawson DM, Courdavault V, O'Connor SE Nat Commun. 2016 Jul 15;7:12116. doi: 10.1038/ncomms12116. PMID:27418042<ref>PMID:27418042</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5h81" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Catharanthus roseus]]
[[Category: Large Structures]]
[[Category: Caputi L]]
[[Category: Courdavault V]]
[[Category: Foureau E]]
[[Category: Lawson DM]]
[[Category: O'Connor SE]]
[[Category: Stavrinides A]]
[[Category: Stevenson CEM]]
[[Category: Tatsis EC]]