View source for 5hhl


==Reverse transcriptase domain of group II intron maturase from Eubacterium rectale in P21 space group==
<StructureSection load='5hhl' size='340' side='right'caption='[[5hhl]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5hhl]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Eubacterium_rectale_M104/1 Eubacterium rectale M104/1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HHL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5HHL FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=K:POTASSIUM+ION'>K</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5hhl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5hhl OCA], [https://pdbe.org/5hhl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5hhl RCSB], [https://www.ebi.ac.uk/pdbsum/5hhl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5hhl ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/D4JMT6_9FIRM D4JMT6_9FIRM] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Group II introns are self-splicing ribozymes that are essential in many organisms, and they have been hypothesized to share a common evolutionary ancestor with the spliceosome. Although structural similarity of RNA components supports this connection, it is of interest to determine whether associated protein factors also share an evolutionary heritage. Here we present the crystal structures of reverse transcriptase (RT) domains from two group II intron-encoded proteins (maturases) from Roseburia intestinalis and Eubacterium rectale, obtained at 1.2-A and 2.1-A resolution, respectively. These domains are more similar in architecture to the spliceosomal Prp8 RT-like domain than to any other RTs, and they share substantial similarity with flaviviral RNA polymerases. The RT domain itself is sufficient for binding intron RNA with high affinity and specificity, and it is contained within an active RT enzyme. These studies provide a foundation for understanding structure-function relationships within group II intron-maturase complexes.

Crystal structures of a group II intron maturase reveal a missing link in spliceosome evolution.,Zhao C, Pyle AM Nat Struct Mol Biol. 2016 May 2. doi: 10.1038/nsmb.3224. PMID:27136328<ref>PMID:27136328</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5hhl" style="background-color:#fffaf0;"></div>
== References ==
<references/>
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</StructureSection>
[[Category: Large Structures]]
[[Category: Pyle AM]]
[[Category: Zhao C]]