View source for 5hk0


==Crystal structure of M. tuberculosis MazF-mt3 (Rv1991c) in complex with RNA==
<StructureSection load='5hk0' size='340' side='right'caption='[[5hk0]], [[Resolution|resolution]] 2.25&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5hk0]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis] and [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_H37Rv Mycobacterium tuberculosis H37Rv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HK0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5HK0 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.25&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5hk0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5hk0 OCA], [https://pdbe.org/5hk0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5hk0 RCSB], [https://www.ebi.ac.uk/pdbsum/5hk0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5hk0 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/MAZF6_MYCTU MAZF6_MYCTU] Toxic component of a type II toxin-antitoxin (TA) module. Upon expression in E.coli and in M.smegmatis partially inhibits cell growth and colony formation; its toxic effect is neutralized by coexpression with cognate antitoxin MazE6. Acts as an mRNA interferase on ssRNA, cleaving between the second and third bases in the sequences CUCCU and UUCCU (PubMed:18485066). Further experiments demonstrate that it digests between the first and second bases of UCCUU, yielding a 5'-hydroxyl end; digests M.tuberculosis mRNA (in coding as well as the 5'- and 3'-UTR regions) and 23S rRNA, digests E.coli 16S rRNA both alone and in the 70S ribosome but no data for M.tuberculosis 16S rRNA cleavage was presented. 23S and 16S rRNA digestion occurs in predicted single-stranded regions, the 16S rRNA UCCUU site is in the anti-Shine-Dalgarno site and would cleave off the last 7 nucleotides (PubMed:24709835). Non-cognate antitoxins VapB27 and VapB40 partially neutralize toxicity in vivo (PubMed:20876537).<ref>PMID:17623030</ref> <ref>PMID:18258191</ref> <ref>PMID:18485066</ref> <ref>PMID:19016878</ref> <ref>PMID:20011113</ref> <ref>PMID:20876537</ref> <ref>PMID:24709835</ref> <ref>PMID:25608501</ref> 
== References ==
<references/>
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</StructureSection>
[[Category: Large Structures]]
[[Category: Mycobacterium tuberculosis]]
[[Category: Mycobacterium tuberculosis H37Rv]]
[[Category: Brennan RG]]
[[Category: Yen TJ]]