View source for 5hmb


==Crystal structure of S. sahachiroi AziG==
<StructureSection load='5hmb' size='340' side='right'caption='[[5hmb]], [[Resolution|resolution]] 2.15&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5hmb]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_sahachiroi Streptomyces sahachiroi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HMB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5HMB FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.151&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5hmb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5hmb OCA], [https://pdbe.org/5hmb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5hmb RCSB], [https://www.ebi.ac.uk/pdbsum/5hmb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5hmb ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/B4XYA6_STREG B4XYA6_STREG] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The azinomycins are a family of potent antitumor agents with the ability to form interstrand cross-links with DNA. This study reports on the unusual biosynthetic formation of the 5-methyl naphthoate moiety, which is essential for effective DNA association. While sequence analysis predicts that the polyketide synthase (AziB) catalyzes the formation of this naphthoate, 2-methylbenzoic acid, a truncated single-ring product, is formed instead. We demonstrate that the thioesterase (AziG) acts as a chain elongation and cyclization (CEC) domain and is required for the additional two rounds of chain extension to form the expected product.

Polyketide Ring Expansion Mediated by a Thioesterase, Chain Elongation and Cyclization Domain, in Azinomycin Biosynthesis: Characterization of AziB and AziG.,Mori S, Simkhada D, Zhang H, Erb MS, Zhang Y, Williams H, Fedoseyenko D, Russell WK, Kim D, Fleer N, Ealick SE, Watanabe CM Biochemistry. 2016 Feb 2;55(4):704-14. doi: 10.1021/acs.biochem.5b01050. Epub, 2016 Jan 19. PMID:26731610<ref>PMID:26731610</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5hmb" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Streptomyces sahachiroi]]
[[Category: Ealick SE]]
[[Category: Erb MS]]
[[Category: Zhang Y]]