View source for 5hok


==MamB-CTD mutant - D247A==
<StructureSection load='5hok' size='340' side='right'caption='[[5hok]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5hok]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Magnetospira_sp._QH-2 Magnetospira sp. QH-2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HOK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5HOK FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5hok FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5hok OCA], [https://pdbe.org/5hok PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5hok RCSB], [https://www.ebi.ac.uk/pdbsum/5hok PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5hok ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/MAMB_MAGSQ MAMB_MAGSQ] Plays a dual, essential role in magnetosome formation; required for magnetosome vesicle formation as well as biomineralization (By similarity). Probably binds and transports iron (Probable). Requires heterodimerization with MamM for stability (By similarity).[UniProtKB:V6F510]<ref>PMID:29243866</ref> 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Magnetospirillum gryphiswaldense MSR-1 synthesizes membrane-enclosed magnetite (Fe3 O4 ) nanoparticles, magnetosomes, for magnetotaxis. Formation of these organelles involves a complex process comprising key steps which are governed by specific magnetosome-associated proteins. MamB, a cation diffusion facilitator (CDF) family member has been implicated in magnetosome-directed iron transport. However, deletion mutagenesis studies revealed that MamB is essential for the formation of magnetosome membrane vesicles, but its precise role remains elusive. In this study, we employed a multi-disciplinary approach to define the role of MamB during magnetosome formation. Using site-directed mutagenesis complemented by structural analyses, fluorescence microscopy and cryo-electron tomography, we show that MamB is most likely an active magnetosome-directed transporter serving two distinct, yet essential functions. First, MamB initiates magnetosome vesicle formation in a transport-independent process, probably by serving as a landmark protein. Second, MamB transport activity is required for magnetite nucleation. Furthermore, by determining the crystal structure of the MamB cytosolic C-terminal domain, we also provide mechanistic insight into transport regulation. Additionally, we present evidence that magnetosome vesicle growth and chain formation are independent of magnetite nucleation and magnetic interactions respectively. Together, our data provide novel insight into the role of the key bifunctional magnetosome protein MamB, and the early steps of magnetosome formation.

The dual role of MamB in magnetosome membrane assembly and magnetite biomineralization.,Uebe R, Keren-Khadmy N, Zeytuni N, Katzmann E, Navon Y, Davidov G, Bitton R, Plitzko JM, Schuler D, Zarivach R Mol Microbiol. 2018 Feb;107(4):542-557. doi: 10.1111/mmi.13899. Epub 2018 Jan 9. PMID:29243866<ref>PMID:29243866</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5hok" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Magnetospira sp. QH-2]]
[[Category: Keren N]]
[[Category: Zarivach R]]
[[Category: Zeytuni N]]