View source for 5hyx


==Plant peptide hormone receptor RGFR1 in complex with RGF1==
<StructureSection load='5hyx' size='340' side='right'caption='[[5hyx]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5hyx]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis Arabidopsis] and [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HYX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5HYX FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.596&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HZP:(4S)-4-HYDROXY-L-PROLINE'>HZP</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=PTR:O-PHOSPHOTYROSINE'>PTR</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5hyx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5hyx OCA], [https://pdbe.org/5hyx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5hyx RCSB], [https://www.ebi.ac.uk/pdbsum/5hyx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5hyx ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/RGI3_ARATH RGI3_ARATH] Together with RGI1, RGI2, RGI4 and RGI5, acts as a receptor of RGF peptides (e.g. RGF1, GLV5/CLEL1/RGF2, GLV7/CLEL3/RGF3, GLV3/RGF4, GLV10/CLEL7/RGF5 and RGF10/CLELN), peptide hormones which maintain the postembryonic root stem cell niche by regulating the expression levels and patterns of the transcription factor PLETHORA (PLT, e.g. PLT1 and PLT2) (PubMed:27001831, PubMed:27229311, PubMed:27229312). Links RGF peptides signal with their downstream components (PubMed:27001831, PubMed:27229311).<ref>PMID:27001831</ref> <ref>PMID:27229311</ref> <ref>PMID:27229312</ref> 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Peptide-mediated cell-to-cell signaling has crucial roles in coordination and definition of cellular functions in plants. Peptide-receptor matching is important for understanding the mechanisms underlying peptide-mediated signaling. Here we report the structure-guided identification of root meristem growth factor (RGF) receptors important for plant development. An assay based on a signature ligand recognition motif (Arg-x-Arg) conserved in a subfamily of leucine-rich repeat receptor kinases (LRR-RKs) identified the functionally uncharacterized LRR-RK At4g26540 as a receptor of RGF1 (RGFR1). We further solved the crystal structure of RGF1 in complex with the LRR domain of RGFR1 at a resolution of 2.6 A, which reveals that the Arg-x-Gly-Gly (RxGG) motif is responsible for specific recognition of the sulfate group of RGF1 by RGFR1. Based on the RxGG motif, we identified additional four RGFRs. Participation of the five RGFRs in RGF-induced signaling is supported by biochemical and genetic data. We also offer evidence showing that SERKs function as co-receptors for RGFs. Taken together, our study identifies RGF receptors and co-receptors that can link RGF signals with their downstream components and provides a proof of principle for structure-based matching of LRR-RKs with their peptide ligands.

Signature motif-guided identification of receptors for peptide hormones essential for root meristem growth.,Song W, Liu L, Wang J, Wu Z, Zhang H, Tang J, Lin G, Wang Y, Wen X, Li W, Han Z, Guo H, Chai J Cell Res. 2016 Jun;26(6):674-85. doi: 10.1038/cr.2016.62. Epub 2016 May 27. PMID:27229311<ref>PMID:27229311</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5hyx" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Arabidopsis]]
[[Category: Arabidopsis thaliana]]
[[Category: Large Structures]]
[[Category: Chai J]]
[[Category: Han Z]]
[[Category: Song W]]