View source for 5hzu


==Crystal structure of Dronpa-Ni2+==
<StructureSection load='5hzu' size='340' side='right'caption='[[5hzu]], [[Resolution|resolution]] 1.89&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5hzu]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Echinophyllia_sp._SC22 Echinophyllia sp. SC22]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HZU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5HZU FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.89&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GYS:[(4Z)-2-(1-AMINO-2-HYDROXYETHYL)-4-(4-HYDROXYBENZYLIDENE)-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL]ACETIC+ACID'>GYS</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5hzu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5hzu OCA], [https://pdbe.org/5hzu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5hzu RCSB], [https://www.ebi.ac.uk/pdbsum/5hzu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5hzu ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q5TLG6_9CNID Q5TLG6_9CNID] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Many fluorescent proteins (FPs) show fluorescence quenching by specific metal ions, which can be applied towards metal biosensor development. In this study, we investigated the significant fluorescence quenching of Dronpa by Co(2+) and Cu(2+) ions. Crystal structures of Co(2+) -, Ni(2+) - and Cu(2+) -bound Dronpa revealed previously unseen, unique, metal-binding sites for fluorescence quenching. These metal ions commonly interact with surface-exposed histidine residues (His194-His210 and His210-His212), and interact indirectly with chromophores. Structural analysis of the Co(2+) - and Cu(2+) - binding sites of Dronpa provides insight into FP-based metal biosensor engineering.

Crystal structures of Dronpa complexed with quenchable metal ions provide insight into metal biosensor development.,Kim IJ, Kim S, Park J, Eom I, Kim S, Kim JH, Ha SC, Kim YG, Hwang KY, Nam KH FEBS Lett. 2016 Sep;590(17):2982-90. doi: 10.1002/1873-3468.12316. Epub 2016 Jul , 30. PMID:27433793<ref>PMID:27433793</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5hzu" style="background-color:#fffaf0;"></div>

==See Also==
*[[Dronpa|Dronpa]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Echinophyllia sp. SC22]]
[[Category: Large Structures]]
[[Category: Hwang KY]]
[[Category: Nam KH]]