View source for 5ida


==Crystal structure of CGL1 from Crassostrea gigas, mannose-bound form (CGL1/MAN)==
<StructureSection load='5ida' size='340' side='right'caption='[[5ida]], [[Resolution|resolution]] 1.10&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5ida]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Crassostrea_gigas Crassostrea gigas]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5IDA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5IDA FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.1&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AYA:N-ACETYLALANINE'>AYA</scene>, <scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5ida FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ida OCA], [https://pdbe.org/5ida PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5ida RCSB], [https://www.ebi.ac.uk/pdbsum/5ida PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5ida ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/K1QRB6_MAGGI K1QRB6_MAGGI] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
A novel mannose-specific lectin, named CGL1 (15.5 kDa), was isolated from the oyster Crassostrea gigas. Characterization of CGL1 involved isothermal titration calorimetry (ITC), glycoconjugate microarray, and frontal affinity chromatography (FAC). This analysis revealed that CGL1 has strict specificity for the mannose monomer and for high mannose-type N-glycans (HMTGs). Primary structure of CGL1 did not show any homology with known lectins but did show homology with proteins of the natterin family. Crystal structure of the CGL1 revealed a unique homodimer in which each protomer was composed of 2 domains related by a pseudo two-fold axis. Complex structures of CGL1 with mannose molecules showed that residues have 8 hydrogen bond interactions with O1, O2, O3, O4, and O5 hydroxyl groups of mannose. The complex interactions that are not observed with other mannose-binding lectins revealed the structural basis for the strict specificity for mannose. These characteristics of CGL1 may be helpful as a research tool and for clinical applications.

Identification, Characterization, and X-ray Crystallographic Analysis of a Novel Type of Mannose-Specific Lectin CGL1 from the Pacific Oyster Crassostrea gigas.,Unno H, Matsuyama K, Tsuji Y, Goda S, Hiemori K, Tateno H, Hirabayashi J, Hatakeyama T Sci Rep. 2016 Jul 5;6:29135. doi: 10.1038/srep29135. PMID:27377186<ref>PMID:27377186</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5ida" style="background-color:#fffaf0;"></div>
== References ==
<references/>
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</StructureSection>
[[Category: Crassostrea gigas]]
[[Category: Large Structures]]
[[Category: Unno H]]