View source for 5ii0


==Crystal structure of the human calcitonin receptor ectodomain in complex with a truncated salmon calcitonin analogue==
<StructureSection load='5ii0' size='340' side='right'caption='[[5ii0]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5ii0]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Oncorhynchus_gorbuscha Oncorhynchus gorbuscha]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5II0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5II0 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.097&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=4BF:4-BROMO-L-PHENYLALANINE'>4BF</scene>, <scene name='pdbligand=LPD:L-PROLINAMIDE'>LPD</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=URE:UREA'>URE</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5ii0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ii0 OCA], [https://pdbe.org/5ii0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5ii0 RCSB], [https://www.ebi.ac.uk/pdbsum/5ii0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5ii0 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/CALCR_HUMAN CALCR_HUMAN] This is a receptor for calcitonin. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase. The calcitonin receptor is thought to couple to the heterotrimeric guanosine triphosphate-binding protein that is sensitive to cholera toxin.
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Calcitonin is a peptide hormone consisting of 32 amino acid residues and the calcitonin receptor is a Class B G protein-coupled receptor (GPCR). The crystal structure of the human calcitonin receptor ectodomain (CTR ECD) in complex with a truncated analogue of salmon calcitonin ([BrPhe22]sCT(8-32)) has been determined to 2.1 A resolution. Parallel analysis of a series of peptide ligands showed that the rank order of binding of the CTR ECD is identical to the rank order of binding of the full length CTR, confirming the structural integrity and relevance of the isolated CTR ECD. The structure of the CTR ECD is similar to other Class B GPCRs and the ligand binding site is similar to the binding site of the homologous receptors for the calcitonin gene-related peptide (CGRP) and adrenomedulin (AM) recently published. Interestingly the receptor-bound structure of the ligand [BrPhe22]sCT(8-32) differs from the receptor-bound structure of the homologous ligands CGRP and AM. They all adopt an extended conformation followed by a carboxy terminal beta turn, however, [BrPhe22]sCT(8-32) adopts a type II turn (Gly28-Thr31) whereas CGRP and AM adopt type I turns. Our results suggest that a type II turn is the preferred conformation of calcitonin whereas a type I turn is the preferred conformation of peptides that require receptor activity-modifying proteins (RAMPs); CGRP, AM and amylin. In addition the structure provides a detailed molecular explanation and hypothesis regarding ligand binding properties of CTR and the amylin receptors.

Type II Turn of Receptor-bound Salmon Calcitonin Revealed by X-ray Crystallography.,Johansson E, Hansen JL, Hansen AM, Shaw AC, Becker P, Schaffer L, Reedtz-Runge S J Biol Chem. 2016 May 4. pii: jbc.M116.726034. PMID:27189946<ref>PMID:27189946</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5ii0" style="background-color:#fffaf0;"></div>
== References ==
<references/>
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Oncorhynchus gorbuscha]]
[[Category: Johansson E]]
[[Category: Reedtz-Runge S]]