View source for 5iml


==Nanobody targeting human Vsig4 in Spacegroup P212121==
<StructureSection load='5iml' size='340' side='right'caption='[[5iml]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5iml]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Camelidae Camelidae] and [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5IML OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5IML FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5iml FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5iml OCA], [https://pdbe.org/5iml PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5iml RCSB], [https://www.ebi.ac.uk/pdbsum/5iml PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5iml ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/VSIG4_HUMAN VSIG4_HUMAN] Phagocytic receptor, strong negative regulator of T-cell proliferation and IL2 production. Potent inhibitor of the alternative complement pathway convertases.<ref>PMID:17016562</ref> <ref>PMID:17051150</ref> 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Vsig4 is a recently identified immune regulatory protein related to the B7 family with dual functionality: a negative regulator of T cell activation and a receptor for the complement components C3b and C3c. Here we present a structural evaluation of a nanobody, Nb119, against the extracellular IgV domain protein of both mouse and human recombinant Vsig4, which have a high degree of sequence identity. Although mouse and human Vsig4 bind to Nb119 with a 250 times difference in dissociation constants, the interaction results in a highly identical assembly with a RMSD of 0.4A. The molecular determinants for Vsig4 recognition and cross reactivity unveiled by the atomic structure of Nb119 in complex with mVsig4 and hVsig4 afford new insights useful for the further optimization of the nanobody for potential use in humans. Additionally, structural analysis of the Vsig4-Nb119 complexes indicates that Nb119 occupies the interface on Vsig4 recognized by the macroglobulin-like domains MG4 and MG5 of C3b. Thus an affinity-improved Nb119 may have the potential to influence the activation of both T cells and complement.

Structural evaluation of a nanobody targeting complement receptor Vsig4 and its cross reactivity.,Wen Y, Ouyang Z, Schoonooghe S, Luo S, De Baetselier P, Lu W, Muyldermans S, Raes G, Zheng F Immunobiology. 2016 Nov 18. pii: S0171-2985(16)30436-3. doi:, 10.1016/j.imbio.2016.11.008. PMID:27889311<ref>PMID:27889311</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5iml" style="background-color:#fffaf0;"></div>
== References ==
<references/>
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</StructureSection>
[[Category: Camelidae]]
[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Wen Y]]