View source for 5ip2


==Tomato spotted wilt tospovirus nucleocapsid protein-ssRNA complex==
<StructureSection load='5ip2' size='340' side='right'caption='[[5ip2]], [[Resolution|resolution]] 3.30&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5ip2]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Tomato_spotted_wilt_orthotospovirus Tomato spotted wilt orthotospovirus] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5IP2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5IP2 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.3&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5ip2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ip2 OCA], [https://pdbe.org/5ip2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5ip2 RCSB], [https://www.ebi.ac.uk/pdbsum/5ip2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5ip2 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/F4ZD19_TSWV F4ZD19_TSWV] Encapsidates the RNA.[PIRNR:PIRNR003948]
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== Publication Abstract from PubMed ==
Tomato spotted wilt virus (TSWV), belonging to the genus Tospovirus of the family Bunyaviridae, causes significant economic damage to several vegetables and ornamental plants worldwide. Similar to those of all other negative-strand RNA viruses, the nucleocapsid (N) protein plays very important roles in its viral life cycle. N proteins protect genomic RNAs by encapsidation and form a viral ribonucleoprotein complex (vRNP) with some RNA-dependent RNA polymerases. Here we show the crystal structure of the N protein from TSWV. Protomers of TSWV N proteins consist of three parts: the N arm, C arm, and core domain. Unlike N proteins of other negative-strand RNA viruses, the TSWV N protein forms an asymmetric trimeric ring. To form the trimeric ring, the N and C arms of the N protein interact with the core domains of two adjacent N proteins. By solving the crystal structures of the TSWV N protein with nucleic acids, we showed that an inner cleft of the asymmetric trimeric ring is an RNA-binding site. These characteristics are similar to those of N proteins of other viruses of the family Bunyaviridae Based on these observations, we discuss possibilities of a TSWV encapsidation model.IMPORTANCE Tospoviruses cause significant crop losses throughout the world. Particularly, TSWV has an extremely wide host range (&gt;1,000 plant species, including dicots and monocots), and worldwide losses are estimated to be in excess of $1 billion annually. Despite such importance, no proteins of tospoviruses have been elucidated so far. Among TSWV-encoded proteins, the N protein is required for assembling the viral genomic RNA into the viral ribonucleoprotein (vRNP), which is involved in various steps of the life cycle of these viruses, such as RNA replication, virus particle formation, and cell-to-cell movement. This study revealed the structure of the N protein, with or without nucleic acids, of TSWV as the first virus of the genus Tospovirus, so it completed our view of the N proteins of the family Bunyaviridae.

Asymmetric Trimeric Ring Structure of the Nucleocapsid Protein of Tospovirus.,Komoda K, Narita M, Yamashita K, Tanaka I, Yao M J Virol. 2017 Sep 27;91(20). pii: JVI.01002-17. doi: 10.1128/JVI.01002-17. Print , 2017 Oct 15. PMID:28768868<ref>PMID:28768868</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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==See Also==
*[[Nucleoprotein 3D structures|Nucleoprotein 3D structures]]
== References ==
<references/>
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</StructureSection>
[[Category: Large Structures]]
[[Category: Synthetic construct]]
[[Category: Tomato spotted wilt orthotospovirus]]
[[Category: Komoda K]]
[[Category: Narita M]]
[[Category: Tanaka I]]
[[Category: Yamashita K]]
[[Category: Yao M]]