View source for 5iya


==Human core-PIC in the closed state==
<SX load='5iya' size='340' side='right' viewer='molstar' caption='[[5iya]], [[Resolution|resolution]] 5.40&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5iya]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5IYA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5IYA FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 5.4&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5iya FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5iya OCA], [https://pdbe.org/5iya PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5iya RCSB], [https://www.ebi.ac.uk/pdbsum/5iya PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5iya ProSAT]</span></td></tr>
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== Function ==
[https://www.uniprot.org/uniprot/T2EA_HUMAN T2EA_HUMAN] Recruits TFIIH to the initiation complex and stimulates the RNA polymerase II C-terminal domain kinase and DNA-dependent ATPase activities of TFIIH. Both TFIIH and TFIIE are required for promoter clearance by RNA polymerase.
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
In eukaryotic transcription initiation, a large multi-subunit pre-initiation complex (PIC) that assembles at the core promoter is required for the opening of the duplex DNA and identification of the start site for transcription by RNA polymerase II. Here we use cryo-electron microscropy (cryo-EM) to determine near-atomic resolution structures of the human PIC in a closed state (engaged with duplex DNA), an open state (engaged with a transcription bubble), and an initially transcribing complex (containing six base pairs of DNA-RNA hybrid). Our studies provide structures for previously uncharacterized components of the PIC, such as TFIIE and TFIIH, and segments of TFIIA, TFIIB and TFIIF. Comparison of the different structures reveals the sequential conformational changes that accompany the transition from each state to the next throughout the transcription initiation process. This analysis illustrates the key role of TFIIB in transcription bubble stabilization and provides strong structural support for a translocase activity of XPB.

Near-atomic resolution visualization of human transcription promoter opening.,He Y, Yan C, Fang J, Inouye C, Tjian R, Ivanov I, Nogales E Nature. 2016 May 11;533(7603):359-65. doi: 10.1038/nature17970. PMID:27193682<ref>PMID:27193682</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 5iya" style="background-color:#fffaf0;"></div>

==See Also==
*[[Helicase 3D structures|Helicase 3D structures]]
*[[RNA polymerase 3D structures|RNA polymerase 3D structures]]
*[[TATA-binding protein 3D structures|TATA-binding protein 3D structures]]
*[[Transcription initiation factors 3D structures|Transcription initiation factors 3D structures]]
== References ==
<references/>
__TOC__
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[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Fang J]]
[[Category: He Y]]
[[Category: Inouye C]]
[[Category: Ivanov I]]
[[Category: Nogales E]]
[[Category: Tjian R]]
[[Category: Yan C]]