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==The Structure and Mechanism of NOV1, a Resveratrol-Cleaving Dioxygenase==
<StructureSection load='5j55' size='340' side='right'caption='[[5j55]], [[Resolution|resolution]] 1.75&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5j55]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Novosphingobium_aromaticivorans_DSM_12444 Novosphingobium aromaticivorans DSM 12444]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5J55 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5J55 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.75&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=OXY:OXYGEN+MOLECULE'>OXY</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene>, <scene name='pdbligand=V55:4-HYDROXY-3-METHOXYBENZALDEHYDE'>V55</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5j55 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5j55 OCA], [https://pdbe.org/5j55 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5j55 RCSB], [https://www.ebi.ac.uk/pdbsum/5j55 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5j55 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q2GA76_NOVAD Q2GA76_NOVAD] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Stilbenes are diphenyl ethene compounds produced naturally in a wide variety of plant species and some bacteria. Stilbenes are also derived from lignin during kraft pulping. Stilbene cleavage oxygenases (SCOs) cleave the central double bond of stilbenes, forming two phenolic aldehydes. Here, we report the structure of an SCO. The X-ray structure of NOV1 from Novosphingobium aromaticivorans was determined in complex with its substrate resveratrol (1.89 A), its product vanillin (1.75 A), and without any bound ligand (1.61 A). The enzyme is a seven-bladed beta-propeller with an iron cofactor coordinated by four histidines. In all three structures, dioxygen is observed bound to the iron in a side-on fashion. These structures, along with EPR analysis, allow us to propose a mechanism in which a ferric-superoxide reacts with substrate activated by deprotonation of a phenol group at position 4 of the substrate, which allows movement of electron density toward the central double bond and thus facilitates reaction with the ferric superoxide electrophile. Correspondingly, NOV1 cleaves a wide range of other stilbene-like compounds with a 4'-OH group, offering potential in processing some solubilized fragments of lignin into monomer aromatic compounds.

Structure and mechanism of NOV1, a resveratrol-cleaving dioxygenase.,McAndrew RP, Sathitsuksanoh N, Mbughuni MM, Heins RA, Pereira JH, George A, Sale KL, Fox BG, Simmons BA, Adams PD Proc Natl Acad Sci U S A. 2016 Dec 13;113(50):14324-14329. Epub 2016 Nov 30. PMID:27911781<ref>PMID:27911781</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5j55" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Novosphingobium aromaticivorans DSM 12444]]
[[Category: Adams PD]]
[[Category: McAndrew RP]]
[[Category: Pereira JH]]
[[Category: Sale KL]]
[[Category: Sathitsuksanoh N]]
[[Category: Simmons BA]]