View source for 5jeu


==del-[Ru(phen)2(dppz)]2+ bound to d(TCGGCGCCGA) with Ba2+==
<StructureSection load='5jeu' size='340' side='right'caption='[[5jeu]], [[Resolution|resolution]] 0.97&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5jeu]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5JEU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5JEU FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 0.97&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=0TN:DELTA-RU(PHEN)2(DPPZ)+COMPLEX'>0TN</scene>, <scene name='pdbligand=BA:BARIUM+ION'>BA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5jeu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5jeu OCA], [https://pdbe.org/5jeu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5jeu RCSB], [https://www.ebi.ac.uk/pdbsum/5jeu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5jeu ProSAT]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
[Ru(phen)2(dppz)]2+ has been studied since the 1990s due to its 'light-switch' properties. It can be used as a luminescent DNA probe, with emission switched on through DNA binding. The luminescence observed is dependent on the solvent accessibility of the pyrazine nitrogen atoms, and therefore is sensitive to changes in both binding site of the cation and chromophore orientation. The compound is also chiral, and there are distinct differences between the enantiomers in terms of the emission behaviour when bound to a variety of DNA sequences. Whilst a number of binary DNA-complex X-ray crystal structures are available, most include the Lambda enantiomer and there is very little structural information about binding of the Delta enantiomer. Here, we present the first X-ray crystal structure of a Delta enantiomer bound to well-matched DNA, in the absence of the other, Lambda enantiomer. We show how the binding site observed here can be related to a more general pattern of motifs in the crystallographic literature and propose that the Delta enantiomer can bind with five different binding modes, offering a new hypothesis for the interpretation of solution data.

Delta chirality ruthenium 'light-switch' complexes can bind in the minor groove of DNA with five different binding modes.,Hall JP, Keane PM, Beer H, Buchner K, Winter G, Sorensen TL, Cardin DJ, Brazier JA, Cardin CJ Nucleic Acids Res. 2016 Sep 5. pii: gkw753. PMID:27599841<ref>PMID:27599841</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5jeu" style="background-color:#fffaf0;"></div>
== References ==
<references/>
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</StructureSection>
[[Category: Large Structures]]
[[Category: Synthetic construct]]
[[Category: Cardin CJ]]
[[Category: Hall JP]]