View source for 5jjf


==Structure of the SRII/HtrII Complex in I212121 space group ("U" shape) - M state==
<StructureSection load='5jjf' size='340' side='right'caption='[[5jjf]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5jjf]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Natronomonas_pharaonis Natronomonas pharaonis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5JJF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5JJF FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BOG:B-OCTYLGLUCOSIDE'>BOG</scene>, <scene name='pdbligand=LFA:EICOSANE'>LFA</scene>, <scene name='pdbligand=RET:RETINAL'>RET</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5jjf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5jjf OCA], [https://pdbe.org/5jjf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5jjf RCSB], [https://www.ebi.ac.uk/pdbsum/5jjf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5jjf ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/BACS2_NATPH BACS2_NATPH] Involved in the control of phototaxis. Seems to activate a methyl-accepting protein (HTR-II). Photoreceptor for blue light.
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The complex of two membrane proteins, sensory rhodopsin II (NpSRII) with its cognate transducer (NpHtrII), mediates negative phototaxis in halobacteria N. pharaonis. Upon light activation NpSRII triggers a signal transduction chain homologous to the two-component system in eubacterial chemotaxis. Here we report on crystal structures of the ground and active M-state of the complex in the space group I212121. We demonstrate that the relative orientation of symmetrical parts of the dimer is parallel ("U"-shaped) contrary to the gusset-like ("V"-shaped) form of the previously reported structures of the NpSRII/NpHtrII complex in the space group P21212, although the structures of the monomers taken individually are nearly the same. Computer modeling of the HAMP domain in the obtained "V"- and "U"-shaped structures revealed that only the "U"-shaped conformation allows for tight interactions of the receptor with the HAMP domain. This is in line with existing data and supports biological relevance of the "U" shape in the ground state. We suggest that the "V"-shaped structure may correspond to the active state of the complex and transition from the "U" to the "V"-shape of the receptor-transducer complex can be involved in signal transduction from the receptor to the signaling domain of NpHtrII.

New Insights on Signal Propagation by Sensory Rhodopsin II/Transducer Complex.,Ishchenko A, Round E, Borshchevskiy V, Grudinin S, Gushchin I, Klare JP, Remeeva A, Polovinkin V, Utrobin P, Balandin T, Engelhard M, Buldt G, Gordeliy V Sci Rep. 2017 Feb 6;7:41811. doi: 10.1038/srep41811. PMID:28165484<ref>PMID:28165484</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5jjf" style="background-color:#fffaf0;"></div>

==See Also==
*[[Bacteriorhodopsin 3D structures|Bacteriorhodopsin 3D structures]]
*[[Rhodopsin 3D structures|Rhodopsin 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Natronomonas pharaonis]]
[[Category: Balandin T]]
[[Category: Borshchevskiy V]]
[[Category: Bueldt G]]
[[Category: Engelhard M]]
[[Category: Gordeliy V]]
[[Category: Grudinin S]]
[[Category: Gushchin I]]
[[Category: Ishchenko A]]
[[Category: Klare J]]
[[Category: Polovinkin V]]
[[Category: Remeeva A]]
[[Category: Round E]]
[[Category: Utrobin P]]