View source for 5jki


==Crystal structure of the first transmembrane PAP2 type phosphatidylglycerolphosphate phosphatase from Bacillus subtilis==
<StructureSection load='5jki' size='340' side='right'caption='[[5jki]], [[Resolution|resolution]] 2.25&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5jki]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis_subsp._subtilis_str._168 Bacillus subtilis subsp. subtilis str. 168]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5JKI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5JKI FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.25&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=OLC:(2R)-2,3-DIHYDROXYPROPYL+(9Z)-OCTADEC-9-ENOATE'>OLC</scene>, <scene name='pdbligand=WO4:TUNGSTATE(VI)ION'>WO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5jki FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5jki OCA], [https://pdbe.org/5jki PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5jki RCSB], [https://www.ebi.ac.uk/pdbsum/5jki PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5jki ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/PGPB_BACSU PGPB_BACSU] Catalyzes the dephosphorylation of phosphatidylglycerophosphate (PGP) to phosphatidylglycerol. Also has undecaprenyl pyrophosphate phosphatase activity, required for the biosynthesis of the lipid carrier undecaprenyl phosphate.<ref>PMID:28168443</ref> 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Type 2 phosphatidic acid phosphatases (PAP2s) can be either soluble or integral membrane enzymes. In bacteria, integral membrane PAP2s play major roles in the metabolisms of glycerophospholipids, undecaprenyl-phosphate (C55-P) lipid carrier and lipopolysaccharides. By in vivo functional experiments and biochemical characterization we show that the membrane PAP2 coded by the Bacillus subtilis yodM gene is the principal phosphatidylglycerol phosphate (PGP) phosphatase of B. subtilis. We also confirm that this enzyme, renamed bsPgpB, has a weaker activity on C55-PP. Moreover, we solved the crystal structure of bsPgpB at 2.25 A resolution, with tungstate (a phosphate analog) in the active site. The structure reveals two lipid chains in the active site vicinity, allowing for PGP substrate modeling and molecular dynamic simulation. Site-directed mutagenesis confirmed the residues important for substrate specificity, providing a basis for predicting the lipids preferentially dephosphorylated by membrane PAP2s.

Crystal structure and biochemical characterization of the transmembrane PAP2 type phosphatidylglycerol phosphate phosphatase from Bacillus subtilis.,Ghachi ME, Howe N, Auger R, Lambion A, Guiseppi A, Delbrassine F, Manat G, Roure S, Peslier S, Sauvage E, Vogeley L, Rengifo-Gonzalez JC, Charlier P, Mengin-Lecreulx D, Foglino M, Touze T, Caffrey M, Kerff F Cell Mol Life Sci. 2017 Feb 6. doi: 10.1007/s00018-017-2464-6. PMID:28168443<ref>PMID:28168443</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5jki" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Bacillus subtilis subsp. subtilis str. 168]]
[[Category: Large Structures]]
[[Category: Auger R]]
[[Category: Caffrey M]]
[[Category: Delbrassine F]]
[[Category: El Ghachi M]]
[[Category: Foglino M]]
[[Category: Guiseppe A]]
[[Category: Howe N]]
[[Category: Lampion A]]
[[Category: Mengin-lecreulx D]]
[[Category: Perlier S]]
[[Category: Roure S]]
[[Category: Sauvage E]]
[[Category: Touze T]]
[[Category: Vogeley L]]