View source for 5jt8


==Structural basis for the limited antibody cross reactivity between the mite allergens Blo t 1 and Der p 1==
<StructureSection load='5jt8' size='340' side='right'caption='[[5jt8]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5jt8]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Blomia_tropicalis Blomia tropicalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5JT8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5JT8 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=CSO:S-HYDROXYCYSTEINE'>CSO</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=NO3:NITRATE+ION'>NO3</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5jt8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5jt8 OCA], [https://pdbe.org/5jt8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5jt8 RCSB], [https://www.ebi.ac.uk/pdbsum/5jt8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5jt8 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/CYSP_BLOTA CYSP_BLOTA] Cysteine protease.[UniProtKB:A5HII1]
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The Blomia tropicalis (Blo t) mite species is considered a storage mite in temperate climate zones and an important source of indoor allergens causing allergic asthma and rhinitis in tropical and subtropical regions. Here, we report the crystal structure of one of the allergens from Blo t, recombinant proBlo t 1 (rproBlo t 1), determined at 2.1 A resolution. Overall, the fold of rproBlo t 1 is characteristic for the pro-form of cysteine proteases from the C1A class. Structural comparison of experimentally mapped Der f 1/Der p1 IgG epitopes to the same surface patch on Blo t 1, as well as of sequence identity of surface-exposed residues, suggests limited cross-reactivity between these allergens and Blo t 1. This is in agreement with ELISA inhibition results showing that, although cross-reactive human IgE epitopes exist, there are unique IgE epitopes for both Blo t 1 and Der p 1.

The structure of the mite allergen Blo t 1 explains the limited antibody cross-reactivity to Der p 1.,Meno KH, Kastrup JS, Kuo IC, Chua KY, Gajhede M Allergy. 2017 Apr;72(4):665-670. doi: 10.1111/all.13111. Epub 2017 Jan 19. PMID:27997997<ref>PMID:27997997</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5jt8" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Blomia tropicalis]]
[[Category: Large Structures]]
[[Category: Gajhede M]]
[[Category: Kastrup JS]]
[[Category: Meno K]]