View source for 5jyk


==Deg9 crystal under 289K==
<StructureSection load='5jyk' size='340' side='right'caption='[[5jyk]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5jyk]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5JYK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5JYK FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.297&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5jyk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5jyk OCA], [https://pdbe.org/5jyk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5jyk RCSB], [https://www.ebi.ac.uk/pdbsum/5jyk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5jyk ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/DEGP9_ARATH DEGP9_ARATH] Probable serine protease.
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The high temperature requirement A (HtrA) proteases (also termed Deg proteases) play important roles in diverse organisms by regulating protein quality and quantity. One of the 16 Arabidopsis homologs, Deg9, is located in the nucleus where it modulates cytokinin- and light-mediated signalling via degrading the ARABIDOPSIS RESPONSE REGULATOR 4 (ARR4). To uncover the structural features underlying the proteolytic activity of Deg9, we determined its crystal structure. Unlike the well-established trimeric building block of HtrAs, Deg9 displays a novel octameric structure consisting of two tetrameric rings that have distinct conformations. Based on the structural architecture, we generated several mutant variants of Deg9, determined their structure and tested their proteolytic activity towards ARR4. The results of the structural and biochemical analyses allowed us to propose a model for a novel mechanism of substrate recognition and activity regulation of Deg9. In this model, protease activation of one tetramer is mediated by en-bloc reorientation of the protease domains to open an entrance for the substrate in the opposite (inactive) tetramer. This study provides the structural basis for understanding how the levels of nuclear signal components are regulated by a plant protease.

The crystal structure of Deg9 reveals a novel octameric-type HtrA protease.,Ouyang M, Li X, Zhao S, Pu H, Shen J, Adam Z, Clausen T, Zhang L Nat Plants. 2017 Dec;3(12):973-982. doi: 10.1038/s41477-017-0060-2. Epub 2017 Nov, 27. PMID:29180814<ref>PMID:29180814</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5jyk" style="background-color:#fffaf0;"></div>
== References ==
<references/>
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</StructureSection>
[[Category: Arabidopsis thaliana]]
[[Category: Large Structures]]
[[Category: Ouyang M]]
[[Category: Zhang LX]]