View source for 5k79


==Structure and anti-HIV activity of CYT-CVNH, a new cyanovirin-n homolog==
<StructureSection load='5k79' size='340' side='right'caption='[[5k79]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5k79]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Gloeothece_citriformis_PCC_7424 Gloeothece citriformis PCC 7424]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5K79 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5K79 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5k79 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5k79 OCA], [https://pdbe.org/5k79 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5k79 RCSB], [https://www.ebi.ac.uk/pdbsum/5k79 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5k79 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/B7KDN5_GLOC7 B7KDN5_GLOC7] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The HIV-1 envelope glycoprotein gp120 is heavily glycosylated and bears numerous high-mannose sugars. These sugars can serve as targets for HIV-inactivating compounds, such as antibodies and lectins, which bind to the glycans and interfere with viral entry into the target cell. We determined the 1.6 A X-ray structure of Cyt-CVNH, a recently identified lectin from the cyanobacterium Cyanothece7424, and elucidated its glycan specificity by NMR. The Cyt-CVNH structure and glycan recognition profile are similar to those of other CVNH proteins, with each domain specifically binding to Mana(1-2)Mana units on the D1 and D3 arms of high-mannose glycans. However, in contrast to CV-N, no cross-linking and precipitation of the cross-linked species in solutions was observed upon Man-9 binding, allowing for the first time to investigate the interaction of Man-9 with a member of the CVNH family by NMR. HIV assays showed that Cyt-CVNH is able to inhibit HIV-1 with ~ 4-fold higher potency than CV-NP51G, a stabilized version of wild type CV-N. Therefore, Cyt-CVNH may qualify as a valuable lectin for potential microbicidal use.

Structure and Glycan Binding of a New Cyanovirin-N Homolog.,Matei E, Basu R, Furey W, Shi J, Calnan C, Aiken C, Gronenborn AM J Biol Chem. 2016 Jul 7. pii: jbc.M116.740415. PMID:27402833<ref>PMID:27402833</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5k79" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Gloeothece citriformis PCC 7424]]
[[Category: Large Structures]]
[[Category: Aiken C]]
[[Category: Basu R]]
[[Category: Calnan C]]
[[Category: Furey W]]
[[Category: Gronenborn AM]]
[[Category: Matei E]]
[[Category: Shi J]]